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OriGene proteins in recent publications

Fully functional proteins purified from transient TrueORF cDNA clone transfections.  Researchers purified GEN1 protein using anti-FLAG M2 affinity column and determined it resolved Holliday junction X0 efficiently in vitro, thus confirming GEN1 is human Holliday junction resolvase.

Identification of Holliday junction resolvases from humans and yeast, Stephen C. Y. Ip, Ulrich Rass, Miguel G. Blanco, Helen R. Flynn, J. Mark Skehel, Stephen C. West, Nature 456, 357 - 361 (20 Nov 2008), doi: 10.1038/nature07470,


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LIF (NM_002309) Purified Human Protein

Specifications Citations Customer Service Product Documents
Cat. No. Description Datasheet Price Availability*  
TP750008 Recombinant protein of human leukemia inhibitory factor (LIF) produced in E. coli., 20 ug (Larger pack size?) ¥3791.00 现货
TA50011-100 4C5, Anti-DDK monoclonal antibody (100 μL) ¥1000 现货
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OriGene Data
Species:Human Expression Host:E. coli
Expression cDNA Clone or AA Sequence
A DNA sequence from TrueORF clone, RC210818, encoding the region (Ser23-Phe202) of human LIF
Tag:Tag-free Predicted MW:19.8 kDa
Purity:>95% as determined by SDS-PAGE and Coomassie blue staining
Concentration:Resuspend the protein to the desired concentration in proper buffer.
Buffer:Lyophilized from a sterile solution containing 20 mM phosphate buffer, pH7.4. Stable for at least 6 months from date of receipt under proper storage and handling conditions.
Endotoxin: <0.1 EU per 1 ?g of the protein by the LAL
BioActivity: Determined by its ability to induce differentiation of M1 cells. ED50 for this effect is typically < 0.04 ng/ml.
Protein Families: Secreted Protein
ES Cell Differentiation/IPS
Adult stem cells
Druggable Genome
Protein Pathways: Cytokine-cytokine receptor interaction
Jak-STAT signaling pathway
Protein Data
  Protein Image
 

Reference Data
RefSeq: NP_002300 RefSeq Size: 3868 RefSeq ORF: 609
LocusID: 3976 Cytogenetic: 22q12.2  
Synonyms : CDF; DIA; HILDA; MLPLI
Summary: The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012].
*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact techsupport@origene.com for specific inventory information
**: DDK-tag is the same as FLAG tag. Flag? is a registered trademark of Sigma-Aldrich

 

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