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OriGene proteins in recent publications

Fully functional proteins purified from transient TrueORF cDNA clone transfections.  Researchers purified GEN1 protein using anti-FLAG M2 affinity column and determined it resolved Holliday junction X0 efficiently in vitro, thus confirming GEN1 is human Holliday junction resolvase.

Identification of Holliday junction resolvases from humans and yeast, Stephen C. Y. Ip, Ulrich Rass, Miguel G. Blanco, Helen R. Flynn, J. Mark Skehel, Stephen C. West, Nature 456, 357 - 361 (20 Nov 2008), doi: 10.1038/nature07470,


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HIST3H3 (NM_003493) Purified Human Protein

Specifications Citations Customer Service Product Documents
Cat. No. Description Datasheet Price Availability*  
TP750003 Recombinant protein of human Histone H3 (HIST3H3) produced in E. coli., 250 ?g (Larger pack size?) ¥3791.00 现货
TA50011-100 4C5, Anti-DDK monoclonal antibody (100 μL) ¥1000 现货
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Also for HIST3H3 (NM_003493)

OriGene Data
Species:Human Expression Host:E. coli
Expression cDNA Clone or AA Sequence
A DNA sequence from TrueORF clone, RC219443, encoding the region (Met1-Ala13polyhistidinetone H3
Tag:Tag-free Predicted MW:15.5 kDa
Purity:>95% as determined by SDS-PAGE and Coomassie blue staining
Concentration:Resuspend the protein to the desired concentration in proper buffer.
Buffer:Lyophilized from a sterile solution containing 20 mM phosphate buffer, pH7.4. Stable for at least 6 months from date of receipt under proper storage and handling conditions.
Endotoxin: <0.1 EU per 1 ?g of the protein by the LAL
Protein Pathways: Systemic lupus erythematosus
Protein Data
  Protein Image
 

Reference Data
RefSeq: NP_003484 RefSeq Size: 481 RefSeq ORF: 411
LocusID: 8290 Cytogenetic: 1q42.13  
Synonyms : H3.4; H3/g; H3FT; H3t
Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015].
*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact techsupport@origene.com for specific inventory information
**: DDK-tag is the same as FLAG tag. Flag? is a registered trademark of Sigma-Aldrich

 

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