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OriGene proteins in recent publications

Fully functional proteins purified from transient TrueORF cDNA clone transfections.  Researchers purified GEN1 protein using anti-FLAG M2 affinity column and determined it resolved Holliday junction X0 efficiently in vitro, thus confirming GEN1 is human Holliday junction resolvase.

Identification of Holliday junction resolvases from humans and yeast, Stephen C. Y. Ip, Ulrich Rass, Miguel G. Blanco, Helen R. Flynn, J. Mark Skehel, Stephen C. West, Nature 456, 357 - 361 (20 Nov 2008), doi: 10.1038/nature07470,


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FGF2 (NM_002006) Purified Human Protein

Specifications Citations (3) Customer Service Product Documents
Cat. No. Description Datasheet Price Availability*  
TP750002 Recombinant protein of human Fibroblast Growth Factor-basic (FGF2) produced in E. coli, 100 ug (Larger pack size?) ¥3791.00 现货
TA50011-100 4C5, Anti-DDK monoclonal antibody (100 μL) ¥1000 现货
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Also for FGF2 (NM_002006)

OriGene Data
Species:Human Expression Host:E. coli
Expression cDNA Clone or AA Sequence
A DNA sequence from TrueORF clone, RC217426, encoding the region (Gly132-Ser288) of human FGF2
Tag:Tag-free Predicted MW:17.4 kDa
Purity:>95% as determined by SDS-PAGE and Coomassie blue staining
Concentration:Resuspend the protein to the desired concentration in proper buffer.
Buffer:Lyophilized from a sterile solution containing 10 mM Tris.Cl, pH7.0, 150mM NaCl. Stable for at least 6 months from date of receipt under proper storage and handling conditions.
Endotoxin: <0.1 EU per 1 ?g of the protein by the LAL
BioActivity: Measured in a cell proliferation assay using 3T3 cells. ED50 for this effect is typically 0.07-0.10 ng/ml.
Protein Families: Secreted Protein
Druggable Genome
Protein Pathways: MAPK signaling pathway
Regulation of actin cytoskeleton
Pathways in cancer
Melanoma
Protein Data
  Protein Image
 

Reference Data
RefSeq: NP_001997 RefSeq Size: 6803 RefSeq ORF: 867
LocusID: 2247 Cytogenetic: 4q28.1  
Synonyms : BFGF; FGF-2; FGFB; HBGF-2
Summary: The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008].
*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact techsupport@origene.com for specific inventory information
**: DDK-tag is the same as FLAG tag. Flag? is a registered trademark of Sigma-Aldrich

 

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