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OriGene proteins in recent publications

Fully functional proteins purified from transient TrueORF cDNA clone transfections.  Researchers purified GEN1 protein using anti-FLAG M2 affinity column and determined it resolved Holliday junction X0 efficiently in vitro, thus confirming GEN1 is human Holliday junction resolvase.

Identification of Holliday junction resolvases from humans and yeast, Stephen C. Y. Ip, Ulrich Rass, Miguel G. Blanco, Helen R. Flynn, J. Mark Skehel, Stephen C. West, Nature 456, 357 - 361 (20 Nov 2008), doi: 10.1038/nature07470,


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FGF1 (NM_000800) Purified Human Protein

Specifications Citations Customer Service Product Documents
Cat. No. Description Datasheet Price Availability*  
TP750001 Recombinant protein of human Fibroblast Growth Factor-acidic (FGF1) produced in E. coli, 100 ug (Larger pack size?) ¥3791.00 现货
TA50011-100 4C5, Anti-DDK monoclonal antibody (100 μL) ¥1000 现货
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Also for FGF1 (NM_000800)

OriGene Data
Species:Human Expression Host:E. coli
Expression cDNA Clone or AA Sequence
A DNA sequence from TrueORF clone, RC207434, encoding the region (Phe16-Asp155) of human FGF1
Tag:Tag-free Predicted MW:15.9 kDa
Purity:>95% as determined by SDS-PAGE and Coomassie blue staining
Concentration:Resuspend the protein to the desired concentration in proper buffer.
Buffer:Lyophilized from a sterile solution containing 50 mM Tris.Cl, pH7.5, 150mM NaCl. Stable for at least 6 months from date of receipt under proper storage and handling conditions.
Endotoxin: <0.1 EU per 1 ?g of the protein by the LAL
BioActivity: Measured?in?a cell proliferation assay using 3T3 cells. ED50 for?this?effect?is?typically 0.031 - 0.046 ng/ml.
Protein Families: Secreted Protein
Druggable Genome
Protein Pathways: MAPK signaling pathway
Regulation of actin cytoskeleton
Pathways in cancer
Melanoma
Protein Data
  Protein Image
 

Reference Data
RefSeq: NP_000791 RefSeq Size: 2357 RefSeq ORF: 468
LocusID: 2246 Cytogenetic: 5q31.3  
Synonyms : AFGF; ECGF; ECGF-beta; ECGFA; ECGFB; FGF-1; FGF-alpha; FGFA; GLIO703; HBGF-1; HBGF1
Summary: The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009]
*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact techsupport@origene.com for specific inventory information
**: DDK-tag is the same as FLAG tag. Flag? is a registered trademark of Sigma-Aldrich

 

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