Viromer^® – Virus-like polymer, active escape transfection reagent

Viromers, a technology breakthrough, take advantage of a viral fusion mechanism (hence their name) and translate this into polymer chemistry for plasmid and siRNA transfection

• Superior transfection efficiency, active endosome escape, maximizes transfection efficacy and reduces background
• Simplified use, no need to remove serum or antibiotics
• Lipid-free, no interaction with cell metabolism
• Reverse Transfection, ready for High-throughput Screening
• Best for hard-to-transfect cells
     - primary cells,
     - differentiating or stem cells
     - suspension cells
  

Cell Lines Tested       

Plasmid/mRNA Transfection

Plasmid Transfection

siRNA/miRNA Transfection

CRISPR RNP Transfectoin

mRNA Transfection – Viromer mRNA

Plasmid or mRNA Transfection – Viromer Red, Viromer Yellow

Viromer® RED has been extensively optimized for binding of large oligonucleotides such as mRNA and plasmids. By using the innovative Viromer® technology excellent delivery of mRNA or plasmid transfection complexes can be achieved into standard cells like HEK-293, HeLa and CHO as well as in challenging cells such as C2C12 myoblasts , primary macrophages, keratinocytes and pancreatic tumor cells. Very high plasmid transfection efficiency up to 80% can be achieved in primary human macrophages. Based on the fact that primary keratinocytes are predestinated to prevent uptake of any material an outcome of 65% plasmid transfection efficiency in these challenging cells by using Viromer® RED is a fantastic result. Please have a look to our extensive cell-data-base which includes all cell types where transfection using Viromer® results in an efficiency of mRNA/plasmid transfection up to 90%.

Please see cell lines tested.

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Plasmid Transfection – Viromer Plasmid

A Powerful and Potent Plasmid DNA Transfection Reagent

• Virus-like polymer
• Improved plasmid transfection efficiency
• Versatile for standard and challenging cells
• Outperforms major competitors

Viromer Plasmid is superior for plasmid DNA transfection. Viromer Red has been proven to be a great plasmid transfection reagent for many challenging cells, please see cells tested by the customers. Viromer Plasmid has been tested to provide even better transfection efficiency. Please see the data blow.

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siRNA /miRNA Transfection – Viromer Blue & Green

Viromer® Blue & Green are optimized for binding the size of small oligonucleotides like miRNA and siRNA. It is based on a medium sized branched polymer and the neutral charge of Viromer BLUE : miRNA and siRNA transfection complexes results in no aggregation and high performance in suspension cells like primary microglia cells.

Viromer® BLUE gives high miRNA or siRNA transfection results in common cell lines, including HEK-293, HeLa and CHO as well as in challenging primary cells like mesenchymal stem cells, macrophages, monocytes, myoblasts and hepatocytes.

Viromer® GREEN works extremely efficient in the siRNA transfection of fibroblasts, colon carcinoma or in THP-1 transfection. Please see cell lines tested.

• Virus-like Polymer
• Active endosome escape leading to high siRNA / miRNA transfection efficiency
• Gentle on cells and compatible with antibiotics and serum

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CRISPR RNP Transfection – Viromer CRISPR

For genome editing using CRISPR, sometimes Cas9 protein and gRNA complexes (RNP) are preferred to use due to its fast, less target effects and leaving no foot-print.

Viromer CRISPR was developed to transfect CRISPR RNP into cells at high efficiency.

• Virus-like Polymer
• Active endosome escape leading to high CRISPR RNP transfection efficiency
• Low impact on cell viability and physiology
• Easily scalable, easy gRNA screening, suitable for HTS

Why use CRISPR RNP for genome editing?

CRISPR components directly active upon transfection

• No need to use the cell machinery
• Skips the assembly step in the cytosol

Better control of the Cas9 activity

• Adjustable amounts of delivered Cas9 and gRNA
• Rapid protein clearance
• Transient action limiting off-target cleavage
• No integration of Cas9 gene into the cell genome

View more transfection data

mRNA Transfection – Viromer mRNA

• Virus-like polymer
• Active endosome escape leading to high mRNA transfection efficiency
• Best for challenging and primary cells, such as monocytes, macrophages, microglia cells
• Outperforms major competitors

Why use mRNA vs plasmid for gene expression?

Transfecting plasmid DNA is the most common method for gene overexpression in cells; however, in some challenging cells, plasmid transfection efficiency is very low. mRNA transfection has been proven to give higher efficiency.

• mRNA is shorter: leading to higher transfection efficiency
• mRNA is faster and very productive template: used for protein translation, skipping transcription
• mRNA is safer: no risk of genomic integration

mRNA transfection efficiency is much higher in challenging cells

Which Viromer works better for your cells? Try the Viromer samples! Cat# for the Viromer samples, TT100302S (Red), TT100300S (Blue), TT100301S (Green), TT100310 (Viromer CRISPR), TT100312S (Viromer Plasmid), TT100313S (Viromer mRNA)

Free Viromer samples with purchase of any OriGene’s product.

Viromer samples are available for $105 each when ordered alone.

Transfecting Immune cells and Myocytes

Viromer Transfection Data

Viromer Red, plasmid DNA transfection, RAW 264.7

Viromer Red, capped GFP mRNA transfection in primary monocytes

Viromer Plasmid outperforms major competitors

Viromer Blue (siRNA transfection)

P4E6 prostate cancer cells. Red: florescence labelled siRNA

Viromer CRISPR successfully delivered CRISPR RNP into C2C12 and produced 50% genome editing

RNP (fluorescence labeled gRNA) was delivered by Viromer CRISPR. Images taken 24 hrs post transfection. T7E1 assay was used to analyze genome editing efficiency.

RNP delivered by Viromer CRISPR leads to efficient genome editing

CRISPR RNP (HPRT1 sgRNA and Cas9, TP790148) was transfected into A549 cells using Viromer CRISPR. Genome editing efficiency was analyzed with T7E1 assay.

RFP CRISPR RNP (Cas9, TP790148) was delivered in HEK293 RFP stable cells by Viromer CRISPR. Images were taken 5 days post transfection.