pCMV6-AN-FC-S Mammalian Expression Vector

CAT#: PS100056

pCMV6-AN-FC-S, mammalian vector with N-terminal FC and secretion signal, 10ug



CNY 5,420.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

Specifications

Product Data
N-Tag FC
E. coli Selection Ampicillin (100 ug/mL)
Cell Selection Neomycin
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as a secreted tagged protein with an N-terminal murine Fc tag. Due to the presence of an N-terminal secretion signal sequence, the fusion protein will be secreted and this facilitates the downstream purification.
  • The Fc-tag encodes the CH2 and CH3 domains of the mouse IgG heavy chain and the hinge region. A fusion protein is comprised of two relatively independent parts connected with a flexible spacer. Fc fusion provides several advantages in protein applications:
    1. Easy purification using protein A or protein G affinity chromatography.
    2. Increased solubility.
    3. Excellent antigen in mouse mAB production without cleavage, as the murine Fc is not antigenic in mice.
  • If the N-terminal tagging interferes with the protein’s function, you can choose the C-terminal Fc-tagging vector, PS100054.
  • Validation Data
  • FC_F Primer can be used to sequence the target sequence after cloning.
  • XL39_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

Documents

Customer Reviews 
Loading...