Products

Proteins

Primary Antibodies

Expression Plasmids

shRNA Plasmids

Recombinant Proteins

CRISPRa Kits

Native Proteins

qPCR Primer Pairs

siRNA Oligo Duplexes

Knockout Kits (CRISPR)

qPCR Template Standards

Human

Mouse

KRT8

E. coli

HEK293T

C-MYC/DDK

10 mM Tris-HCl

2 mM dithiothreitol

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbecco's PBS).

pH 7.4). For immunization purposes

the solution can be further dialyzed against PBS (phosphate buffered saline

2

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