Products

Proteins

Primary Antibodies

Expression Plasmids

shRNA Plasmids

Recombinant Proteins

CRISPRa Kits

qPCR Primer Pairs

qPCR Template Standards

siRNA Oligo Duplexes

Knockout Kits (CRISPR)

Human

Mouse

KRT19

E. coli

HEK293T

C-MYC/DDK

10 mM Tris-HCl

2 mM dithiothreitol

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).

pH 7.4).
For immunization purposes

the solution can be further dialyzed against PBS (phosphate buffered saline

2

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