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Top2b - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Products
Top2b (tGFP-tagged) - Mouse topoisomerase (DNA) II beta (Top2b)
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
- TrueORF®
Top2b (Myc-DDK-tagged) - Mouse topoisomerase (DNA) II beta (Top2b)
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-AC-Myc-DDK |
- TrueORF®
Lenti ORF clone of Top2b (mGFP-tagged) - Mouse topoisomerase (DNA) II beta (Top2b)
| Type | Mouse Tagged ORF Clone |
| Tag | mGFP |
| Vector | pLenti-C-mGFP-P2A-Puro |
- LentiORF®
Lenti ORF clone of Top2b (Myc-DDK-tagged) - Mouse topoisomerase (DNA) II beta (Top2b)
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Top2b - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Top2b (untagged) - Mouse topoisomerase (DNA) II beta (Top2b), (10ug)
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
Top2b - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
qSTAR qPCR primer pairs against Mus musculus gene Top2b
Top2b (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
