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Strada - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Products
Strada CRISPRa kit - CRISPR gene activation of mouse STE20-related kinase adaptor alpha
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
qPCR primer pairs and template standards against Mus musculus gene 2610019A05Rik
| Application | Plasmid of exact quantity for transcript copy number calculation |
qSTAR qPCR primer pairs against Mus musculus gene Strada
Strada (Myc-DDK-tagged) - Mouse STE20-related kinase adaptor alpha (Strada)
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
Strada (myc-DDK-tagged) - Mouse STE20-related kinase adaptor alpha (Strada), transcript variant 3
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
Strada (myc-DDK-tagged) - Mouse STE20-related kinase adaptor alpha (Strada), transcript variant 1
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
Strada (tGFP-tagged) - Mouse RIKEN cDNA 2610019A05 gene (2610019A05Rik)
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
- TrueORF®
Lenti ORF clone of Strada (Myc-DDK-tagged) - Mouse STE20-related kinase adaptor alpha (Strada)
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Lenti ORF clone of Strada (mGFP-tagged) - Mouse STE20-related kinase adaptor alpha (Strada)
| Type | Mouse Tagged ORF Clone |
| Tag | mGFP |
| Vector | pLenti-C-mGFP-P2A-Puro |
- LentiORF®
Strada (untagged) - Mouse STE20-related kinase adaptor alpha (Strada), transcript variant 1
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
Strada - Mouse, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRFP-C-RS |
Strada (untagged) - Mouse STE20-related kinase adaptor alpha (Strada), (10ug)
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | PCMV6-Kan/Neo |
Strada - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Strada (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
