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Pde1a - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Products
Pde1a CRISPRa kit - CRISPR gene activation of mouse phosphodiesterase 1A, calmodulin-dependent
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Purified recombinant protein of Mouse phosphodiesterase 1A, calmodulin-dependent (Pde1a), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
| Tag | C-MYC/DDK |
| Expression Host | HEK293T |
Pde1a (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
qSTAR qPCR primer pairs against Mus musculus gene Pde1a
Pde1a (Myc-DDK-tagged) - Mouse phosphodiesterase 1A, calmodulin-dependent (Pde1a), transcript variant 1
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
Pde1a (tGFP-tagged) - Mouse phosphodiesterase 1A, calmodulin-dependent (cDNA clone MGC:116577 IMAGE:6842675)
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
- TrueORF®
Pde1a (Myc-DDK-tagged) - Mouse phosphodiesterase 1A, calmodulin-dependent (Pde1a), transcript variant 2
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
Pde1a (tGFP-tagged) - Mouse phosphodiesterase 1A calmodulin-dependent (Pde1a) transcript variant 9, (10ug)
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
- TrueORF®
Pde1a (tGFP-tagged) - Mouse phosphodiesterase 1A calmodulin-dependent (Pde1a) transcript variant 7, (10ug)
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
- TrueORF®
Pde1a (Myc-DDK-tagged) - Mouse phosphodiesterase 1A, calmodulin-dependent (Pde1a), transcript variant 7
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
Pde1a (Myc-DDK-tagged) - Mouse phosphodiesterase 1A, calmodulin-dependent (Pde1a), transcript variant 9
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
Pde1a (tGFP-tagged) - Mouse phosphodiesterase 1A calmodulin-dependent (Pde1a) transcript variant 1, (10ug)
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
- TrueORF®
Lenti ORF clone of Pde1a (Myc-DDK-tagged) - Mouse phosphodiesterase 1A, calmodulin-dependent (Pde1a), transcript variant 2
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Lenti ORF clone of Pde1a (mGFP-tagged) - Mouse phosphodiesterase 1A, calmodulin-dependent (Pde1a), transcript variant 2
| Type | Mouse Tagged ORF Clone |
| Tag | mGFP |
| Vector | pLenti-C-mGFP-P2A-Puro |
- LentiORF®
