Products

TNNT2 CRISPRa kit - CRISPR gene activation of human troponin T2, cardiac type

Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)

TNNT2 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).

Cardiac Troponin T (1-285, His-tag) human recombinant protein, 0.1 mg

Tag His-tag
Expression Host E. coli

Cardiac Troponin T (1-285, His-tag) human recombinant protein, 0.5 mg

Tag His-tag
Expression Host E. coli

3' UTR clone of troponin T type 2 (cardiac) (TNNT2) transcript variant 2 for miRNA target validation

Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase

qPCR primer pairs and template standards against Homo sapiens gene TNNT2

Application Plasmid of exact quantity for transcript copy number calculation

TNNT2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided

Format Retroviral plasmids
Vector pRS

3' UTR clone of troponin T type 2 (cardiac) (TNNT2) transcript variant 4 for miRNA target validation

Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase

3' UTR clone of troponin T type 2 (cardiac) (TNNT2) transcript variant 3 for miRNA target validation

Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase

3' UTR clone of troponin T type 2 (cardiac) (TNNT2) transcript variant 1 for miRNA target validation

Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase