Products

SRM CRISPRa kit - CRISPR gene activation of human spermidine synthase

Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)

Spermidine synthase / SRM (1-302, His-tag) human recombinant protein, 0.1 mg

Tag His-tag
Expression Host E. coli

Spermidine synthase / SRM (1-302, His-tag) human recombinant protein, 0.5 mg

Tag His-tag
Expression Host E. coli

SRM (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).

qPCR primer pairs and template standards against Homo sapiens gene SRM

Application Plasmid of exact quantity for transcript copy number calculation

qPCR primer pairs and template standards against Homo sapiens gene SRM

Application Plasmid of exact quantity for transcript copy number calculation

SRM - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided

Format Retroviral plasmids
Vector pRS

3' UTR clone of spermidine synthase (SRM) for miRNA target validation

Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase