Products

COL4A1 CRISPRa kit - CRISPR gene activation of human collagen type IV alpha 1 chain

Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)

COL4A1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).

COL4A1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided

Format Retroviral plasmids
Vector pRS

Collagen type IV human protein, 0.5 mg

Protein Source Placenta

3' UTR clone of collagen type IV alpha 1 (COL4A1) for miRNA target validation

Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase