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Proteins

Recombinant Proteins

Mouse

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VIM

10 mM sodium phosphate

2 mM dithiothreitol

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

e.g. Dulbecco's PBS).

pH 7.4). For immunization purposes

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (phosphate buffered saline

2