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qPCR primer pairs and template standards against Homo sapiens gene SCFD1
| Application | Plasmid of exact quantity for transcript copy number calculation |
Products
SCFD1 (untagged) - Human sec1 family domain containing 1 (SCFD1), transcript variant 5
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
SCFD1 (untagged) - Homo sapiens sec1 family domain containing 1 (SCFD1), transcript variant 3
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
SCFD1 (untagged) - Human sec1 family domain containing 1 (SCFD1), transcript variant 4
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
SCFD1 (untagged) - Human sec1 family domain containing 1 (SCFD1), transcript variant 6
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
Scfd1 (untagged ORF) - Rat sec1 family domain containing 1 (Scfd1), (10 ug)
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
Purified recombinant protein of Mouse sec1 family domain containing 1 (cDNA clone MGC:38021 IMAGE:5151118), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
| Tag | C-MYC/DDK |
| Expression Host | HEK293T |
SCFD1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
SCFD1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Scfd1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Scfd1 - Rat, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Scfd1 - Rat, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
3' UTR clone of sec1 family domain containing 1 (SCFD1) transcript variant 1 for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
Transient overexpression of SCFD1, transcript variant 1, in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
Scfd1 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
