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3' UTR clone of sphingosine kinase 1 (SPHK1) transcript variant 2 for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
Products
Lenti ORF clone of Sphk1 (mGFP-tagged) - Mouse sphingosine kinase 1 (Sphk1), transcript variant 3
| Type | Mouse Tagged ORF Clone |
| Tag | mGFP |
| Vector | pLenti-C-mGFP-P2A-Puro |
- LentiORF®
Lenti ORF clone of Sphk1 (Myc-DDK-tagged) - Mouse sphingosine kinase 1 (Sphk1), transcript variant 3
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Lenti ORF clone of Sphk1 (Myc-DDK-tagged) - Mouse sphingosine kinase 1 (Sphk1), transcript variant 4
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Lenti ORF clone of Human sphingosine kinase 1 (SPHK1), transcript variant 1, Myc-DDK-tagged
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Lenti ORF clone of Sphk1 (Myc-DDK-tagged) - Mouse sphingosine kinase 1 (Sphk1), transcript variant 1
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Lenti ORF clone of Sphk1 (Myc-DDK-tagged) - Mouse sphingosine kinase 1 (Sphk1), transcript variant 1
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK |
- LentiORF®
Lenti ORF clone of Sphk1 (mGFP-tagged) - Mouse sphingosine kinase 1 (Sphk1), transcript variant 5
| Type | Mouse Tagged ORF Clone |
| Tag | mGFP |
| Vector | pLenti-C-mGFP-P2A-Puro |
- LentiORF®
Lenti ORF clone of Sphk1 (Myc-DDK-tagged) - Mouse sphingosine kinase 1 (Sphk1), transcript variant 5
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Sphk1 (untagged) - Mouse sphingosine kinase 1 (Sphk1), transcript variant 2, (10ug)
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
Transient overexpression lysate of sphingosine kinase 1 (SPHK1), transcript variant 2
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
SPHK1 HEK293T cell transient overexpression lysate (as WB positive control)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
SPHK1 HEK293T cell transient overexpression lysate (as WB positive control)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Sphk1 - Mouse, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRFP-C-RS |
SPHK1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
