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3' UTR clone of E2F transcription factor 1 (E2F1) for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
Products
E2f1 - Mouse, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRFP-C-RS |
E2F1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
qSTAR qPCR primer pairs against Mus musculus gene E2f1
qPCR primer pairs and template standards against Homo sapiens gene E2F1
| Application | Plasmid of exact quantity for transcript copy number calculation |
E2f1 (untagged ORF) - Rat E2F transcription factor 1 (E2f1), (10 ug)
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
E2f1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
E2f1 - Rat, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Transient overexpression of E2F1 in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
E2f1 (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
E2f1 (untagged) - Mouse E2F transcription factor 1 (E2f1), transcript variant 2
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
qSTAR qPCR primer pairs against Homo sapiens gene E2F1
Purified recombinant protein of Mouse E2F transcription factor 1 (E2f1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
| Tag | C-MYC/DDK |
| Expression Host | HEK293T |
E2f1 - Rat, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
