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Wnt5a - mouse gene knockout kit via CRISPR, HDR mediated
| Format | 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control |
| Donor DNA | RFP-BSD |
Products
WNT5A CRISPRa kit - CRISPR gene activation of human Wnt family member 5A
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Wnt5a CRISPRa kit - CRISPR gene activation of mouse wingless-type MMTV integration site family, member 5A
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Transient overexpression lysate of wingless-type MMTV integration site family, member 5A (WNT5A)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
WNT5A MS Standard C13 and N15-labeled recombinant protein (NP_003383)
| Tag | C-Myc/DDK |
| Expression Host | HEK293 |
WNT5A - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pGFP-V-RS |
Wnt5a (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Wnt5a - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pGFP-V-RS |
qPCR primer pairs and template standards against Homo sapiens gene WNT5A
| Application | Plasmid of exact quantity for transcript copy number calculation |
Wnt5a (untagged ORF) - Rat wingless-type MMTV integration site family, member 5A (Wnt5a), (10 ug)
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
WNT5A (untagged) - Homo sapiens wingless-type MMTV integration site family, member 5A (WNT5A), transcript variant 2
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
Wnt5a - Rat, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Wnt5a - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Transient overexpression of WNT5A in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
Wnt5a (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
