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NGLY1 CRISPRa kit - CRISPR gene activation of human N-glycanase 1
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
NGLY1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
NGLY1 HEK293T cell transient overexpression lysate (as WB positive control)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Transient overexpression lysate of N-glycanase 1 (NGLY1), transcript variant 1
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
NGLY1 (untagged)-Human N-glycanase 1 (NGLY1), transcript variant 1
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
Ngly1 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
NGLY1 MS Standard C13 and N15-labeled recombinant protein (NP_060767)
| Tag | C-Myc/DDK |
| Expression Host | HEK293 |
NGLY1 rabbit polyclonal antibody, Serum
| Applications | ELISA, IHC, WB |
| Reactivities | Human |
NGLY1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Ngly1 (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Ngly1 (untagged) - Mouse N-glycanase 1 (Ngly1), (10ug)
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | PCMV6-Kan/Neo |
qPCR primer pairs and template standards against Mus musculus gene Ngly1
| Application | Plasmid of exact quantity for transcript copy number calculation |
NGLY1 (untagged)-Human N-glycanase 1 (NGLY1), transcript variant 3
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6 series |
3' UTR clone of N-glycanase 1 (NGLY1) transcript variant 3 for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
3' UTR clone of N-glycanase 1 (NGLY1) transcript variant 4 for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
