Products

Transient overexpression lysate of N-glycanase 1 (NGLY1), transcript variant 1

Tag C-Myc/DDK
Expression Host HEK293T

NGLY1 rabbit polyclonal antibody, Serum

Applications ELISA, IHC, WB
Reactivities Human

Ngly1 - mouse gene knockout kit via CRISPR, HDR mediated

Format 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
Donor DNA RFP-BSD

Ngly1 - mouse gene knockout kit via CRISPR, HDR mediated

Format 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
Donor DNA mBFP-Neo

Ngly1 - mouse gene knockout kit via CRISPR, HDR mediated

Format 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
Donor DNA Luciferase-Puro

Purified recombinant protein of Mouse N-glycanase 1 (Ngly1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Tag C-MYC/DDK
Expression Host HEK293T

NGLY1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided

Format Lentiviral plasmids
Vector pGFP-C-shLenti

NGLY1 HEK293T cell transient overexpression lysate (as WB positive control)

Tag C-Myc/DDK
Expression Host HEK293T

Ngly1 (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).

Ngly1 (untagged) - Mouse N-glycanase 1 (Ngly1), (10ug)

Type Mouse Untagged Clone
Tag Tag Free
Vector PCMV6-Kan/Neo

NGLY1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided

Format Retroviral plasmids
Vector pRS

qPCR primer pairs and template standards against Mus musculus gene Ngly1

Application Plasmid of exact quantity for transcript copy number calculation

NGLY1 (untagged)-Human N-glycanase 1 (NGLY1), transcript variant 3

Type Human Untagged Clone
Tag Tag Free
Vector pCMV6 series

qPCR primer pairs and template standards against Homo sapiens gene NGLY1

Application Plasmid of exact quantity for transcript copy number calculation

NGLY1 (untagged)-Human N-glycanase 1 (NGLY1), transcript variant 2

Type Human Untagged Clone
Tag Tag Free
Vector pCMV6-Entry