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DHFR CRISPRa kit - CRISPR gene activation of human dihydrofolate reductase
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
Dhfr (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
DHFR MS Standard C13 and N15-labeled recombinant protein (NP_000782)
| Tag | C-Myc/DDK |
| Expression Host | HEK293 |
DHFR (untagged)-Human dihydrofolate reductase (DHFR)
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-AC |
DHFR - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
DHFR (untagged) - Human dihydrofolate reductase (DHFR), transcript variant 3
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
DHFR (untagged) - Human dihydrofolate reductase (DHFR), transcript variant 2
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
qPCR primer pairs and template standards against Homo sapiens gene DHFR
| Application | Plasmid of exact quantity for transcript copy number calculation |
Dhfr (untagged ORF) - Rat dihydrofolate reductase (Dhfr), (10 ug)
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
qPCR primer pairs and template standards against Mus musculus gene Dhfr
| Application | Plasmid of exact quantity for transcript copy number calculation |
Dhfr - Rat, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Dhfr - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Transient overexpression of DHFR in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
qSTAR qPCR primer pairs against Homo sapiens gene DHFR
qSTAR qPCR primer pairs against Mus musculus gene Dhfr
