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ASAH1 CRISPRa kit - CRISPR gene activation of human N-acylsphingosine amidohydrolase 1
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
Asah1 CRISPRa kit - CRISPR gene activation of mouse N-acylsphingosine amidohydrolase 1
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Asah1 (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Asah1 (untagged) - Mouse N-acylsphingosine amidohydrolase 1 (Asah1), (10ug)
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
ASAH1 MS Standard C13 and N15-labeled recombinant protein (NP_004306)
| Tag | C-Myc/DDK |
| Expression Host | HEK293 |
(untagged)-Human cDNA: FLJ21558 fis, clone COL06372
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
ASAH1 (untagged)-Human N-acylsphingosine amidohydrolase (acid ceramidase) 1 (ASAH1), transcript variant 2
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
qPCR primer pairs and template standards against Homo sapiens gene ASAH1
| Application | Plasmid of exact quantity for transcript copy number calculation |
ASAH1 (untagged)-Human N-acylsphingosine amidohydrolase (acid ceramidase) 1 (ASAH1), transcript variant 3
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
qPCR primer pairs and template standards against Homo sapiens gene ASAH1
| Application | Plasmid of exact quantity for transcript copy number calculation |
ASAH1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Asah1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Asah1 - Rat, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Asah1 - Rat, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Transient overexpression of ASAH1, transcript variant 3, in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
