United States
Germany
Japan
United Kingdom
China
PYGL CRISPRa kit - CRISPR gene activation of human glycogen phosphorylase L
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
Lenti ORF clone of Pygl (Myc-DDK-tagged ORF) - Rat phosphorylase, glycogen, liver (Pygl), (10 ug)
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
PYGL HEK293T cell transient overexpression lysate (as WB positive control)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Pygl - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Lenti-ORF clone of PYGL (Myc-DDK-tagged)-Human phosphorylase, glycogen, liver (PYGL), transcript variant 2
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
PYGL (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Lenti ORF clone of Human phosphorylase, glycogen, liver (PYGL), transcript variant 1, Myc-DDK-tagged
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Pygl (untagged) - Mouse liver glycogen phosphorylase (Pygl), (10ug)
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | PCMV6-Kan/Neo |
PYGL MS Standard C13 and N15-labeled recombinant protein (NP_002854)
| Tag | C-Myc/DDK |
| Expression Host | HEK293 |
Glycogen phosphorylase liver form (1-847, His-tag) human recombinant protein, 50 µg
| Tag | His-tag |
| Expression Host | E. coli |
Pygl - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pGFP-V-RS |
qSTAR qPCR primer pairs against Homo sapiens gene PYGL
qPCR primer pairs and template standards against Mus musculus gene Pygl
| Application | Plasmid of exact quantity for transcript copy number calculation |
qPCR primer pairs and template standards against Homo sapiens gene PYGL
| Application | Plasmid of exact quantity for transcript copy number calculation |
Purified recombinant protein of Mouse liver glycogen phosphorylase (Pygl), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
| Tag | C-MYC/DDK |
| Expression Host | HEK293T |
