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Polr1b CRISPRa kit - CRISPR gene activation of mouse polymerase (RNA) I polypeptide B
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
Lenti-ORF clone of POLR1B (Myc-DDK-tagged)-Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 2
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Lenti ORF clone of Polr1b (Myc-DDK-tagged) - Mouse polymerase (RNA) I polypeptide B (Polr1b)
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Lenti ORF clone of Polr1b (mGFP-tagged) - Mouse polymerase (RNA) I polypeptide B (Polr1b)
| Type | Mouse Tagged ORF Clone |
| Tag | mGFP |
| Vector | pLenti-C-mGFP-P2A-Puro |
- LentiORF®
Transient overexpression lysate of polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 1
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
POLR1B HEK293T cell transient overexpression lysate (as WB positive control)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
POLR1B (untagged)-Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 1
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
Polr1b (untagged ORF) - Rat polymerase (RNA) I polypeptide B (Polr1b), (10 ug)
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
POLR1B - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Polr1b - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Polr1b - Rat, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Transient overexpression of POLR1B, transcript variant 1, in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
POLR1B (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Polr1b (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Polr1b (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
