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Idua CRISPRa kit - CRISPR gene activation of mouse iduronidase, alpha-L
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
IDUA (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Lenti ORF clone of Human iduronidase, alpha-L- (IDUA), Myc-DDK-tagged
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
IDUA MS Standard C13 and N15-labeled recombinant protein (NP_000194)
| Tag | C-Myc/DDK |
| Expression Host | HEK293 |
qPCR primer pairs and template standards against Mus musculus gene Idua
| Application | Plasmid of exact quantity for transcript copy number calculation |
Idua - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Transient overexpression of IDUA in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
Idua (untagged) - Rat iduronidase, alpha-L- (Idua)
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
Idua (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
qSTAR qPCR primer pairs against Homo sapiens gene IDUA
qSTAR qPCR primer pairs against Mus musculus gene Idua
3' UTR clone of iduronidase alpha-L- (IDUA) for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
Idua (untagged) - Mouse iduronidase, alpha-L- (Idua), transcript variant 2
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
IDUA - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Idua - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
