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GMDS CRISPRa kit - CRISPR gene activation of human GDP-mannose 4,6-dehydratase
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
Transient overexpression lysate of GDP-mannose 4,6-dehydratase (GMDS)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
GMDS MS Standard C13 and N15-labeled recombinant protein (NP_001491)
| Tag | C-Myc/DDK |
| Expression Host | HEK293 |
GMDS - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
GMDS (untagged)-Human GDP-mannose 4,6-dehydratase (GMDS)
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
GMDS (untagged)-Human GDP-mannose 4,6-dehydratase (GMDS)
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-AC |
Gmds (untagged) - Mouse GDP-mannose 4, 6-dehydratase (cDNA clone MGC:18773 IMAGE:4165509), (10ug)
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | PCMV6-Kan/Neo |
qPCR primer pairs and template standards against Homo sapiens gene GMDS
| Application | Plasmid of exact quantity for transcript copy number calculation |
GMDS (untagged) - Homo sapiens GDP-mannose 4,6-dehydratase (GMDS), transcript variant 2
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
Gmds (untagged ORF) - Rat GDP-mannose 4, 6-dehydratase (Gmds), (10 ug)
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
Gmds - Rat, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Transient overexpression of GMDS in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
GMDS (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Gmds (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Gmds (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
