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DGKI CRISPRa kit - CRISPR gene activation of human diacylglycerol kinase iota
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
Dgki CRISPRa kit - CRISPR gene activation of mouse diacylglycerol kinase, iota
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
DGKI MS Standard C13 and N15-labeled recombinant protein (NP_004708)
| Tag | C-Myc/DDK |
| Expression Host | HEK293 |
DGKI (untagged)-Human diacylglycerol kinase, iota (DGKI)
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6 series |
Dgki (untagged ORF) - Rat diacylglycerol kinase, iota (Dgki), (10 ug)
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
3' UTR clone of diacylglycerol kinase iota (DGKI) for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
Purified recombinant protein of Mouse diacylglycerol kinase, iota (Dgki), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
| Tag | C-MYC/DDK |
| Expression Host | HEK293T |
DGKI - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
DGKI - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Dgki - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Dgki - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Dgki - Rat, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Dgki - Rat, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Transient overexpression of DGKI in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
Dgki (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
