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Lenti ORF clone of Vim (mGFP-tagged) - Mouse vimentin (Vim)
| Type | Mouse Tagged ORF Clone |
| Tag | mGFP |
| Vector | pLenti-C-mGFP-P2A-Puro |
- LentiORF®
Products
Vimentin (VIM) mouse monoclonal antibody, clone VIM 3B4, Purified
| Applications | ELISA, IF, IHC, WB |
| Reactivities | Amphibian, Bovine, Chicken, Human, Monkey, Canine |
Vimentin (VIM) mouse monoclonal antibody, clone VM452, Purified
| Applications | FC, IF, IHC, IP, WB |
| Reactivities | Bovine, Canine, Chicken, Feline, Goat, Human, Porcine |
Vimentin (VIM) mouse monoclonal antibody, clone VIM 3B4, FITC
| Applications | ELISA, FC, IF, IHC, WB |
| Reactivities | Amphibian, Bovine, Canine, Chicken, Human, Monkey |
Vimentin (VIM) mouse monoclonal antibody, clone V9, Purified
| Applications | FC, IF, IHC, WB |
| Reactivities | Chicken, Human, Rat, Sheep, Potoroo |
Vimentin (VIM) mouse monoclonal antibody, clone SPM576, Purified
| Applications | FC, IF, IHC, IP, WB |
| Reactivities | Bovine, Canine, Chicken, Feline, Goat, Human, Porcine |
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Reactivities
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- e.g. Dulbecco's PBS). (4)
- 10 mM sodium phosphate (2)
- 10mM Tris-HCl (2)
- 2 mM dithiothreitol (2)
- 2mM dithiothreitol (2)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above) (2) -
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above) (2) - pH 7.4). For immunization purposes (2)
-
pH 7.4).
For immunization purposes (2) - protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (2)
- protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl (2)
- the solution can be further dialyzed against PBS (Phosphate Buffered Saline (2)
- the solution can be further dialyzed against PBS (phosphate buffered saline (2)
