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HIST1H2BM CRISPRa kit - CRISPR gene activation of human histone cluster 1 H2B family member m
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
Lenti ORF clone of Human histone cluster 1, H2bm (HIST1H2BM), Myc-DDK-tagged
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
HIST1H2BM MS Standard C13 and N15-labeled recombinant protein (NP_003512)
| Tag | C-Myc/DDK |
| Expression Host | HEK293 |
Transient overexpression lysate of histone cluster 1, H2bm (HIST1H2BM)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
HIST1H2BM HEK293T cell transient overexpression lysate (as WB positive control)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
HIST1H2BM - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Hist1h2bm - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Transient overexpression of H2BC14 in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
HIST1H2BM (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Hist1h2bm (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Hist1h2bm (untagged) - Mouse histone cluster 1, H2bm (Hist1h2bm), (10ug)
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
HIST1H2BM (untagged)-Human histone cluster 1, H2bm (HIST1H2BM)
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
qSTAR qPCR primer pairs against Homo sapiens gene HIST1H2BM
qSTAR qPCR primer pairs against Mus musculus gene Hist1h2bm
3' UTR clone of histone cluster 1 H2bm (HIST1H2BM) for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
