Products

Proteins

Recombinant Proteins

Human

VIM

E. coli

e.g. Dulbecco's PBS).

10 mM sodium phosphate

10mM Tris-HCl

2 mM dithiothreitol

2mM dithiothreitol

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

pH 7.4). For immunization purposes

pH 7.4).
For immunization purposes

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

the solution can be further dialyzed against PBS (phosphate buffered saline

2

Yes