Products

Proteins

Recombinant Proteins

Human

Bovine

VIM

2 mM dithiothreitol

the solution can be further dialyzed against PBS (phosphate buffered saline

10 mM Tris-HCl

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).
See References 2 and 3 for more details.

pH 7.4).
For Immunization purposes

ELISA.

10 mM sodium phosphate

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

e.g. Dulbecco's PBS).

e.g. Dulbeccos PBS).

pH 7.4). For immunization purposes

pH 7.4).
For immunization purposes

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

ELISA.
Functional Assays.

2