Products

Proteins

Over-expression Lysates

Human

GFAP

WB

HEK293T

C-Myc/DDK

ES Cell Differentiation/IPS

WB

10 mM Tris-HCl

2 mM dithiothreitol

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).

pH 7.4).
For immunization purposes

the solution can be further dialyzed against PBS (phosphate buffered saline

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