Products

cDNA Clones

Antibodies

Proteins

Expression Plasmids

Primary Antibodies

CytoSections

Recombinant Proteins

Mass Spec Standards

Mutant ORF Clones

cDNA Clone Sets

Human

MU OPIOID RECEPTOR

LGR5

GPR56

CHRM2

PTGER3

GPR64

5HT4 RECEPTOR

ANGIOTENSIN II TYPE 1 RECEPTOR

P2Y6

METABOTROPIC GLUTAMATE RECEPTOR 4

CRF1

SIGMA1 RECEPTOR

GPR17

CANNABINOID RECEPTOR I

CCR7

CX3CR1

CXCR4

ENDOTHELIN B RECEPTOR

GPR85

PAF RECEPTOR

GPR1

METABOTROPIC GLUTAMATE RECEPTOR 1

P2RY5

CD97

HRH1

TSH RECEPTOR

ALPHA 1A ADRENERGIC RECEPTOR

GABA B RECEPTOR 1

GPR126

CALCITONIN RECEPTOR

CCR3

GPR161

CMKLR1

LGR7

P2Y2

5HT7 RECEPTOR

ADENOSINE RECEPTOR A2A

DARC

EDG2

FRIZZLED 6

GPR83

SOMATOSTATIN RECEPTOR 3

CALCIUM SENSING RECEPTOR

CXCR2

CXCR3

FPRL1

SOMATOSTATIN RECEPTOR 5

CCR5

DOPAMINE RECEPTOR D3

G PROTEIN COUPLED RECEPTOR 30

NOCICEPTIN RECEPTOR

RGR

C5R1

DOPAMINE D2 RECEPTOR

GNRHR

GPR48

HRH4

MC2 RECEPTOR

P2Y12

VIP RECEPTOR 1

ADENOSINE A3 RECEPTOR

ALPHA 1B ADRENERGIC RECEPTOR

CCR6

CYSLT1

ENDOTHELIN A RECEPTOR

GHRELIN RECEPTOR

NEUROKININ 1 RECEPTOR

SOMATOSTATIN RECEPTOR 2

BETA 2 ADRENERGIC RECEPTOR

CCR2

GPR35

GPRC5C

HTR2C

LEUKOTRIENE B4 RECEPTOR 2

CCR11

CCR9

EMR2

GPR105

GPR155

GPR172B

GPR86

HRH2

LPHN2

MC4 RECEPTOR

METABOTROPIC GLUTAMATE RECEPTOR 3

METABOTROPIC GLUTAMATE RECEPTOR 5

METABOTROPIC GLUTAMATE RECEPTOR 7

METABOTROPIC GLUTAMATE RECEPTOR 8

PACAP RECEPTOR

THROMBIN RECEPTOR

CHRM1

CXCR5

GPR26

NEUROTENSIN RECEPTOR 1

P2Y9

PARATHYROID HORMONE RECEPTOR 1

5 HT 2A

5HT5A RECEPTOR

ADENOSINE A1 RECEPTOR

C3AR1

Human

Mouse

Rat

Dog

Bovine

Gorilla

Bat

Human (Predicted: Monkey)

Monkey

Pig

Human (Predicted: Monkey

Hamster

Rabbit

Human (Predicted: Mouse

Rabbit)

Gibbon

Human (Expected from sequence similarity: Mouse

Dog)

Horse

Horse)

Cow)

Pig)

Primate

Chimpanzee

Canine

Rat)

Gibbon (Predicted: Monkey)

Human (Predicted: Bovine

Guinea Pig)

Hamster)

Human (Predicted: Xenopus)

Rhesus Monkey)

Bovine)

Chicken

Gibbon (Predicted: Monkey

Guinea Pig

Human (Predicted: Bat

Human (Predicted: Bat)

Human (Predicted: Chimpanzee

Human (Predicted: Rabbit)

Porcine

Rat (Expected from sequence similarity: Dog

Xenopus

Xenopus)

Equine

Horse (Predicted: Monkey

Human (Expected from sequence similarity: Dog)

Human (Predicted: Chicken)

Human (Predicted: Dog)

Macaque

Mouse (Expected from sequence similarity: Rat

Mouse (Predicted: Rat

Mouse)

Orang-Utan (Predicted: Mouse

Sheep

X. tropicalis)

Zebrafish

Zebrafish)

Chicken)

Chimpanzee)

Cow

Cow (Expected from sequence similarity: Dog)

Feline

Gibbon (Predicted: Bat

Gibbon (Predicted: Dog

Gibbon (Predicted: Mouse

Goat

Hamster (Predicted: Monkey

Hamster (Predicted: Mouse

Hamster (Predicted: Rabbit)

Horse (Predicted: Bat)

Horse (Predicted: Bovine)

Horse (Predicted: Hamster)

Horse (Predicted: Mouse

Horse (Predicted: Rat

Human (Expected from sequence similarity: Cow)

Human (Expected from sequence similarity: Dog

Human (Expected from sequence similarity: Rat)

Human (Predicted: Bovine)

Human (Predicted: Chicken

Human (Predicted: Dog

Human (Predicted: Hamster)

Human (Predicted: Mouse)

Human (Predicted: Pig)

Human (Predicted: Rat

Human (Predicted: Zebrafish

Monkey (Predicted: Bat

Monkey (Predicted: Rat

Mouse (Expected from sequence similarity: Human

Mouse (Expected from sequence similarity: Rat)

Mouse (Predicted: Bovine)

Orang-Utan (Predicted: Monkey

Pig (Expected from sequence similarity: Dog

Rat (Expected from sequence similarity: Cow)

Rat (Expected from sequence similarity: Dog)

Rat (Expected from sequence similarity: Mouse

Rat (Predicted: Pig)

Rhesus Monkey

IHC

WB

IF

FC

ELISA

ICC/IF

10k-ChIP

IP

Simple Western

PEP-ELISA

FN

Dot

Electron Microscopy

ICC

Rabbit

Mouse

Goat

Rat

Guinea Pig

HEK293T

HEK293

E. coli

Plasmids

Myc-DDK

mGFP

Tag Free

TurboGFP

C-MYC/DDK

C-Myc/DDK

C-His

N-His

N-His-ABP (Albumin-Binding Protein)

C-Fc

C-HUMAN

N-GST and C-His

N-TRX

pCMV6-Entry

pCMV6-AC-GFP

pLenti-C-Myc-DDK-P2A-Puro

pLenti-C-mGFP-P2A-Puro

pLenti-C-Myc-DDK

pLenti-C-mGFP

pCMV6-XL4

pCMV6-XL5

pCMV6-AC

pCMV6-XL6

PCMV6-Neo

pCMV6-AC-Myc-DDK

Unconjugated

Biotin

HRP

PE

APC

DyLight 488

DyLight 550

DyLight 633

FITC

Phospho-specific

IgG

IgG1

Ig

IgG2a

IgG2b

IgG2c

IgM

IgG1, kappa

Polyclonal

Monoclonal

No

Yes

Indirect

LentiORF

TrueORF

TrueClone

TrueORF Gold, TrueORF

TrueMAB

MVPro

UltraMAB

SDS PAGE

Druggable Genome

Transmembrane

Transcription Factors

WB

Secreted Protein

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

GPCR

Phosphatase

Adult stem cells

Ion Channels: Other

Nuclear Hormone Receptor

Embryonic stem cells

Cancer stem cells

Induced pluripotent stem cells

Ion Channels: Potassium

P450

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Cys-loop Receptors

Stem cell relevant signaling - TGFb/BMP signaling pathway

Ion Channels: Transient receptor potential

Stem cell relevant signaling - Wnt Signaling pathway

Ion Channels: Glutamate Receptors

Ion Channels: Calcium

Ion Channels: Sodium

Ion Channels: ATP Receptors

Ion Channels: Cyclic nucleotide gated

ELISA

SDS-PAGE

Bioactivity

the solution can be further dialyzed against PBS (phosphate buffered saline

10 mM Tris-HCl

2 mM dithiothreitol

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbecco's PBS).

ELISA.
Western Blot.

Flow Cyt

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.

pH 7.4).
For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

e.g. Dulbeccos PBS).

e.g. Dulbeccos PBS).
See References 2 and 3 for more details.

pH 7.4).
For immunization purposes

pH 7.4).
For Immunization purposes

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Can be used as standard in a Sandwich ELISA.

ELISA.
Western blotting.

FACS

Protein standard in 1D and 2D SDS gel electrophoresis.
Immunoassay.
Antigen for immunization.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues

Secreted

Suitable for Immunoblotting (Western or Dot blot)

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/32

000-1/40

000.

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western Blot: 1/500-1/2

000.
Western blot: 1/10

500.
Immunohistochemistry: 1/200-1/1

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA: this protein is suitable for use as a Mouse IgE standard in ELISA assays.

ELISA.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA and Immunohistochemistry as well as other beta galactosidase-avidin/biotin based enzymatic assays. This product has been assayed against 1.0 µg of biotinylated IgG in a standard capture ELISA using OPNG as a substrate for 30 minutes at room temperature.
Recommended Dilutions:
ELISA: 1/8000-1/32000.
Western Blot: 1/500-1/2500.
Immunohistochemistry: 1/200-1/1000.

ELISA and immunohistochemistry as well as other glucose oxidase-antibody based enzymatic assays.
Recommended Dilutions:
ELISA: 1/8

ELISA.
Functional Assays.

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

change growth medium against basal medium (EBM) in the early afternoon]
- change EGF against EBM (for HUVEC: EBM +1-2% FCS)
- incubate 24 h
- change medium again and add factors (growth factors

etc.)
- incubate for 18 h
- add 10 µl 3H-Thymidine solution [0.025 mCi/ml] per well (=0.25 µCi)
- incubate another 6h at 37°C
- Washing steps: (250 µl/well)
PBS 1x
MeOH 2x 5 min
TCA 2x 10 min
H2O 1x
- lyse cells in 250 µl 0.3M NaOH per well
- transfer 2.5 ml ECO Plus into the appropriate scintillation vials
- transfer cell lysats into the scintillation vials
- count by liquid scintillation (ß-counter; Beckmann Instruments)

for immunoprecipitation and for Wwestern blotting.

inhibitors

optimal concentration with heparin (50 μg/ml) is about 12 μg/ml.
Protocol: Thymidine Incorporation with HUVEC
- plate cells with a density at 5-7 x 10e3 cells/well in growth medium (EGM)
- incubate cells over night [if urgent

plate cells in the morning

Neuroactive ligand-receptor interaction

Calcium signaling pathway

Chemokine signaling pathway

Cytokine-cytokine receptor interaction

Vascular smooth muscle contraction

Gap junction

Endocytosis

Melanogenesis

Regulation of actin cytoskeleton

Basal cell carcinoma

Colorectal cancer

Pathways in cancer

Wnt signaling pathway

Long-term depression

Complement and coagulation cascades

Olfactory transduction

Renin-angiotensin system

Long-term potentiation

Taste transduction

Epithelial cell signaling in Helicobacter pylori infection

Axon guidance

Leukocyte transendothelial migration

Autoimmune thyroid disease

GnRH signaling pathway

Huntington's disease

Dilated cardiomyopathy

Parkinson's disease

Human

Neomycin

Puromycin

None

Carrier-free Antibodies

1

2

3

Yes

In Stock