Products

cDNA Clones

Antibodies

Proteins

Expression Plasmids

Primary Antibodies

Recombinant Proteins

CytoSections

Mutant ORF Clones

Mass Spec Standards

Over-expression Lysates

Mutant Proteins

Lentiviral Particles

Native Proteins

Mutant CytoSections

Human

Mouse

Humon

BRCA1

P53

BTK

MLH1

ALK

GLUCOKINASE

KALLIKREIN 8

VILIP1

MENIN

BRCA2

HER2

TUBERIN

MEK1

RET

CD4

EGFR

P16INK4A

MSH2

BETA CATENIN

PD1

CD5

NM23A

FGFR1

PROGESTERONE RECEPTOR

ERCC1

C-JUN

P GLYCOPROTEIN

MEK3

VEGFA

CD44

GLI1

KI67

AMYLOID PRECURSOR PROTEIN

PGP9.5

IL6

ALPHA 1 ANTITRYPSIN

NOTCH3

B RAF

NY-ESO-1

CD31

CREM

PD-L1

TAU

CD99

AKT1

NF2

CTNND1

P95 NBS1

LIPG

THYROGLOBULIN

CD40

G PROTEIN ALPHA S

CONSTITUTIVE ANDROSTANE RECEPTOR

EMA

PCNA

MDM2

CD68

ORNITHINE CARBAMOYLTRANSFERASE

TIM 3

BDNF

ADIPONECTIN

FGFR2

CD63

DYSTROBREVIN ALPHA

PDE9A

CD45

GLUCOCORTICOID RECEPTOR

ST2

NRG1

NKX3.1

CD47

IL17

CTLA4

BCL2

FACTOR I

L1CAM

PI 3 KINASE CLASS 2A

CD33

HNF 4 ALPHA

LKB1

CD147

CD30

B7-1

CD56

ESTROGEN RELATED RECEPTOR GAMMA

WILMS TUMOR PROTEIN

ESTROGEN RECEPTOR 1

SAPK4

TOPOISOMERASE II ALPHA

SERPINB3

TGIF

CD20

ERG

MDMX

MSH6

CARDIAC TROPONIN T

P38

VON WILLEBRAND FACTOR

CNOT4

FACTOR XIIIA

Human

Mouse

Rat

Monkey

Dog

Bovine

Human (Expected from sequence similarity: Mouse

Cow)

Pig

Human (Predicted: Mouse

Canine

Chicken

Rabbit

Bovine)

Dog)

Hamster

Porcine

Primate

Rat)

Gorilla

Pig)

Xenopus

Zebrafish

Bat

Rabbit)

Gibbon

Human (Predicted: Monkey)

Horse

Chimpanzee

Guinea Pig

Sheep

Human (Predicted: Mouse)

Rhesus Monkey)

Cow

X. tropicalis)

Xenopus)

Mouse (Expected from sequence similarity: Rat

Mouse (Predicted: Rat

Human (Predicted: Monkey

Human (Predicted: Rat

Rat (Expected from sequence similarity: Dog

Equine

Human (Expected from sequence similarity: Dog)

Human (Expected from sequence similarity: Dog

Human (Does not react with: Mouse)

Mouse)

Rhesus Monkey

Human (Predicted: Bovine

Horse)

Chicken)

Drosophila

Feline

Human (Predicted: Bovine)

Human (Expected from sequence similarity: Rat

Mouse (Expected from sequence similarity: Human

Macaque

Mammalian

Mouse (Predicted: Rat)

Human (Expected from sequence similarity: Mouse)

Goat

Mouse (Predicted: Bovine)

Hamster)

Monkey)

Human (Expected from sequence similarity: Cow)

Human (Predicted: Bat

Human (Predicted: Rat)

Human (Predicted: Rhesus Monkey)

Rat (Expected from sequence similarity: Dog)

Rat (Expected from sequence similarity: Mouse

Fish

Orang-Utan

Pig (Expected from sequence similarity: Dog

Rat (Expected from sequence similarity: Human

Canis

Gibbon (Predicted: Monkey)

Human (Predicted: Pig

Mouse (Predicted: Chicken

Zebrafish)

Human (Predicted: Dog

Mouse (Predicted: Rhesus Monkey)

X. tropicalis

Yeast

Human (Predicted: Chicken

Human (Predicted: Pig)

Human (Predicted: Chimpanzee)

Mouse (Predicted: Bovine

Mouse (Predicted: Pig

Gibbon (Predicted: Monkey

Guinea Pig)

All Species

Human (Expected from sequence similarity: Pig

Human (Predicted: Bat)

Human (Predicted: Dog)

Human (Predicted: Rabbit)

Mouse (Expected from sequence similarity: Dog

Mouse (Expected from sequence similarity: Rat)

Rat (Expected from sequence similarity: Cow)

Rat (Predicted: Mouse

Frog

Gerbil

Horse (Predicted: Mouse

Human (Expected from sequence similarity: Rat)

Human (Predicted: Xenopus)

Opossum

Chimpanzee)

Expected from sequence similarity: Human

Horse (Predicted: Monkey

Human (Expected from sequence similarity: Pig)

Human (Predicted: Chicken)

Human (Predicted: Chimpanzee

Human (Predicted: Cow)

Human (Predicted: Hamster)

Mouse (Predicted: Chimpanzee

Mouse (Predicted: Chimpanzee)

Mouse (Predicted: Dog

Mouse (Predicted: Monkey)

Birds

Gibbon (Predicted: Mouse

Hamster (Predicted: Monkey

Horse (Predicted: Bovine

Horse (Predicted: Monkey)

Human (Expected from sequence similarity: Feline

Insect

Murine

Orang-Utan (Predicted: Monkey)

Pig (Expected from sequence similarity: Rat

Rat (Expected from sequence similarity: Pig

Rat (Predicted: Bovine

African clawed frog

Amphibian

Avian

Baboon

Brugia malayi

C. elegans)

Clostridium botulinum

Deer

Ferret

Gibbon (Predicted: Bat

Gibbon (Predicted: Dog

Horse (Predicted: Bat

Horse (Predicted: Xenopus)

Human (Predicted: Rabbit

Human (Predicted: Xenopus

Human (Predicted: Zebrafish

Invertebrate

Mouse (Expected from sequence similarity: Dog)

Mouse (Expected from sequence similarity: Human)

Mouse (Predicted: Dog)

Mouse (Predicted: Monkey

Mouse (Predicted: Pig)

Mouse (Predicted: Rabbit)

Orang-Utan (Predicted: Mouse

Pig (Expected from sequence similarity: Dog)

Plant

Quail

Rat (Expected from sequence similarity: Mouse)

Saccharomyces cerevisiae

Turkey

Algal

Bonito

Broad

C. elegans

C. elegans (Does not react with: Human

Chicken (weak)

Chlamydomonas

Coral

Cow (Expected from sequence similarity: Dog)

Cow (Expected from sequence similarity: Mouse

Dog (Expected from sequence similarity: Human

Dog (Expected from sequence similarity: Mouse

Dog (Expected from sequence similarity: Rat

Drosophila (weak)

Drosophila)

Escherichia coli

Eukaryote

Feline)

Fungi

Fungus

Gibbon (Predicted: Hamster)

Gibbon (Predicted: Mouse)

Golden Syrian Hamster

Guinea Pig (Predicted: Bat

Guinea Pig (Predicted: Bat)

Guinea Pig (Predicted: Hamster)

Guinea Pig (Predicted: Mouse

Hamster (Predicted: Bat

Hamster (Predicted: Dog

Hamster (Predicted: Mouse

Hamster (Predicted: Mouse)

Hamster (Predicted: Rabbit)

Hamster (Predicted: Rat

Helicobacter pylori

Horse (Predicted: Bat)

Horse (Predicted: Bovine)

Horse (Predicted: Dog

Horse (Predicted: Hamster)

Horse (Predicted: Rabbit

Horse (Predicted: Rat

Human (Predicted: Cow

Human (Predicted: Feline

Human (Predicted: Goat

Human (Predicted: Guinea Pig

Human (Predicted: Hamster

Human (Predicted: Horse

Human (Predicted: X. tropicalis)

Medaka fish)

Monkey (Predicted: Bat

Monkey (Predicted: Dog

Monkey (Predicted: Horse)

Monkey (Predicted: Rat

Mouse (Does not react with: Human)

Mouse (Does not react with: Rabbit)

Mouse (Does not react with: Rat)

Mouse (Predicted: Chicken)

Mouse (Predicted: Feline

Mouse (Predicted: Feline)

Mouse (Predicted: Rabbit

Mouse (Predicted: Xenopus

Mouse (Predicted: Zebrafish

Mouse (Predicted: Zebrafish)

Mouse (weak)

Mustelid

Nicotiana

Orang-Utan (Predicted: Bat

Orang-Utan (Predicted: Bovine

Orang-Utan (Predicted: Hamster

Orang-Utan (Predicted: Horse)

Orang-Utan (Predicted: Monkey

Orang-Utan (Predicted: Zebrafish)

Pig (Expected from sequence similarity: Cow)

Pig (Expected from sequence similarity: Feline

Pig (Expected from sequence similarity: Human

Pig (Predicted: Bovine

Porcine (Does not react with: Mouse)

Predicted: Human

Primate (Does not react with: Mouse)

Primate)

Rabbit (Expected from sequence similarity: Pig

Rat (Does not react with: Human

Rat (Does not react with: Human)

Rat (Expected from sequence similarity: Cow

Rat (Predicted: Bovine)

Rat (Predicted: Chimpanzee)

Rat (Predicted: Monkey

Rat (Predicted: Mouse)

Rat (Predicted: Pig)

Rat (Predicted: Rabbit)

Salmonella Typhimurium

Sheep)

Spinach

Squirrel

Tobacco hornworm

Trichinella spiralis

Tuna

White Fly

Xenopus (weak)

Yeast (Does not react with: Xenopus)

WB

IHC

IF

FC

ELISA

IP

ICC/IF

LMNX

10k-ChIP

Simple Western

FN

Neutralize

Dot

ChIP

PEP-ELISA

R

ID

Immunoblotting

CyTOF-ready

SISCAPA

ICC

Block/Neutralize

Electron Microscopy

SDS-PAGE

E

EMSA

IA

Ie

LF

Labeling

P

Protein Array

RNAi

Sandwich ELISA

Mouse

Rabbit

Goat

Rat

Sheep

Chicken

Guinea Pig

Human

Hamster

HEK293T

HEK293

E. coli

Sf9

Insect

CHO

Baculovirus

Pichia

Plasmids

Particles

Myc-DDK

Tag Free

mGFP

C-Myc/DDK

TurboGFP

C-MYC/DDK

C-His

N-His

C-Fc

N-GST and C-His

C-DDK

C-6His

C-DDK/His

DDK-His

N-GST and C-HIS

N-GST

His-tag

N-His-ABP (Albumin-Binding Protein)

C-Fc&His

C-6His-Avi

C-HIS

C-mFc

N-His&C-His

N-6His

C-8His

C-Human Fc

N-His-Trx

N-Trx&His

C-10His

C-6×His

C-hFc

C-Mouse Fc

His-Tag

C-Fc-Avi

N-GST&His

N-His&SUMO

C-Avi&6His

N-Fc

Tag-free

C-Avi-6His

C-Mouse Fc and 6×His

C- 6×His

C-6xHis

N-HIS

N-His&C-Fc

N-His&T7

N-MARI

N-T7&C-His

N-Trx

C-Fc&AVI

C-Human Fc and 6×His

C-hFc&His

N-8His

N-His/DDK

C-Fc/His

C-Flag

C-HUMAN

N-6His-Avi

N-GST, C-His

N-His&Flag

N-His-PDCD1(Pro21-Val170)

N-Mouse Fc and C-6×His

C-6xHis/AVI

C-AVI&His

C-AVI-PLUS

C-Avi

C-Avi-10His

C-DDK/10xHis

C-DDK/6xHis

C-FC

C-FC-6HIS&C-FC-Flag

C-FLAG-Fc

C-Fc-Flag&C-Fc-6His

C-Flag,C-6His

C-GST

C-HIS/DDK

C-Myc-DDK

C-hIgG2 Fc

C-hIgG4 Fc

C-mFC

N-6His-FLAG

N-6His-Flag

N-AVI

N-AVI&His

N-Avi

N-Avi&6His

N-DDK

N-DDK/His

N-FC

N-FLAG

N-FUSION

N-Fc-FLAG

N-Flag&C-His

N-Flag&His

N-GFP

N-GST(CKM) and N-His(CKB)

N-His-GST

N-Human Fc

N-T7

N-TRX

N-TRXA

N-hFc

N-mFc

N-terminal His

Tag free

pCMV6-Entry

pCMV6-AC-GFP

pLenti-C-Myc-DDK-P2A-Puro

pLenti-C-mGFP-P2A-Puro

pLenti-C-Myc-DDK

pLenti-C-mGFP

pCMV6-XL5

pCMV6-XL4

pCMV6-AC

pCMV6-XL6

pCMV6-AC-Myc-DDK

PCMV6-Neo

pCMV6-XL5-DDK-His

pCMV6-AN-Myc-DDK

pRMT-tGFP, Promoter-less GFP Reporter Vector

KO Validated Antibodies

Unconjugated

Biotin

HRP

FITC

PE

APC

Peroxidase

PerCP

DyLight 488

TRITC

APC-Cy7

ATTO 655

Cy5

DyLight 550

DyLight 633

Magnetic Beads

PE-Cy5.5

PE-Cy7

PE-TXRD

Phospho-specific

IgG1

IgG

IgG2a

IgG2b

Ig

IgG1, kappa

IgG3

IgM

IgG2b, kappa

IgY

IgG2a, kappa

IgG2c

IgG1, lambda

IgM, kappa

IgG3, kappa

IgG, kappa

IgG1/IgK

Monoclonal

Polyclonal

No

Yes

Indirect

Sandwich

Direct

TrueMAB

LentiORF

TrueORF

TrueClone

MVPro

TrueORF Gold, TrueORF

UltraMAB

TrueRAB

SDS PAGE

Druggable Genome

Transmembrane

Transcription Factors

WB

Secreted Protein

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

GPCR

Phosphatase

Adult stem cells

Ion Channels: Other

Nuclear Hormone Receptor

Embryonic stem cells

Cancer stem cells

Induced pluripotent stem cells

Ion Channels: Potassium

P450

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Cys-loop Receptors

Stem cell relevant signaling - TGFb/BMP signaling pathway

Ion Channels: Transient receptor potential

Stem cell relevant signaling - Wnt Signaling pathway

Ion Channels: Glutamate Receptors

Ion Channels: Calcium

Ion Channels: Sodium

Ion Channels: ATP Receptors

Ion Channels: Cyclic nucleotide gated

ELISA

SDS-PAGE

Bioactivity

the solution can be further dialyzed against PBS (phosphate buffered saline

10 mM Tris-HCl

2 mM dithiothreitol

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbecco's PBS).

ELISA.
Western Blot.

Flow Cyt

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.

pH 7.4).
For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

e.g. Dulbeccos PBS).

e.g. Dulbeccos PBS).
See References 2 and 3 for more details.

pH 7.4).
For immunization purposes

pH 7.4).
For Immunization purposes

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Can be used as standard in a Sandwich ELISA.

ELISA.
Western blotting.

FACS

Protein standard in 1D and 2D SDS gel electrophoresis.
Immunoassay.
Antigen for immunization.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues

Secreted

Suitable for Immunoblotting (Western or Dot blot)

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/32

000-1/40

000.

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western Blot: 1/500-1/2

000.
Western blot: 1/10

500.
Immunohistochemistry: 1/200-1/1

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA: this protein is suitable for use as a Mouse IgE standard in ELISA assays.

ELISA.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA and Immunohistochemistry as well as other beta galactosidase-avidin/biotin based enzymatic assays. This product has been assayed against 1.0 µg of biotinylated IgG in a standard capture ELISA using OPNG as a substrate for 30 minutes at room temperature.
Recommended Dilutions:
ELISA: 1/8000-1/32000.
Western Blot: 1/500-1/2500.
Immunohistochemistry: 1/200-1/1000.

ELISA and immunohistochemistry as well as other glucose oxidase-antibody based enzymatic assays.
Recommended Dilutions:
ELISA: 1/8

ELISA.
Functional Assays.

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

change growth medium against basal medium (EBM) in the early afternoon]
- change EGF against EBM (for HUVEC: EBM +1-2% FCS)
- incubate 24 h
- change medium again and add factors (growth factors

etc.)
- incubate for 18 h
- add 10 µl 3H-Thymidine solution [0.025 mCi/ml] per well (=0.25 µCi)
- incubate another 6h at 37°C
- Washing steps: (250 µl/well)
PBS 1x
MeOH 2x 5 min
TCA 2x 10 min
H2O 1x
- lyse cells in 250 µl 0.3M NaOH per well
- transfer 2.5 ml ECO Plus into the appropriate scintillation vials
- transfer cell lysats into the scintillation vials
- count by liquid scintillation (ß-counter; Beckmann Instruments)

for immunoprecipitation and for Wwestern blotting.

inhibitors

optimal concentration with heparin (50 μg/ml) is about 12 μg/ml.
Protocol: Thymidine Incorporation with HUVEC
- plate cells with a density at 5-7 x 10e3 cells/well in growth medium (EGM)
- incubate cells over night [if urgent

plate cells in the morning