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Recombinant protein of human Fibroblast Growth Factor-basic (FGF2) produced in E. coli
| Tag | Tag Free |
| Expression Host | E. coli |
Products
Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E.coli, sterile-filtered, suitable for cell culture
| Tag | N-His |
| Expression Host | E. coli |
Purified fluorescent protein EGFP, with N-terminal HIS tag, expressed in E. coli, 250ug
| Tag | N-His |
| Expression Host | E. coli |
Recombinant protein of human Bone Morphogenetic Protein-2 (BMP2) produced in E. coli.
| Tag | Tag Free |
| Expression Host | E. coli |
Purified recombinant protein of Human nuclear factor (erythroid-derived 2)-like 2 (NFE2L2), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Purified fluorescent protein tdTomato, with N-terminal HIS tag, expressed in E. coli, 250ug
| Tag | N-His |
| Expression Host | E. coli |
Recombinant protein of human Fibroblast Growth Factor-acidic (FGF1) produced in E. coli
| Tag | Tag Free |
| Expression Host | E. coli |
Purified recombinant protein of Human androgen receptor (AR), transcript variant 2, full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug
| Tag | N-GST and C-His |
| Expression Host | E. coli |
Purified recombinant protein of Human RNA binding protein with multiple splicing (RBPMS), transcript variant 3, full length, with N-terminal His tag, expressed in E. coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Recombinant protein of human TRAIL (TNFSF10) produced in E. coli.
| Tag | Tag Free |
| Expression Host | E. coli |
Recombinant protein of human Interleukin 6(IL-6) produced in E. coli.
| Tag | Tag Free |
| Expression Host | E. coli |
Purified recombinant protein of Human NLR family, pyrin domain containing 3 (NLRP3), transcript variant 3, full length, with C-terminal HIS tag, expressed in E.Coli, 50 ug
| Tag | C-HIS |
| Expression Host | E. coli |
Recombinant protein of human Tumor Necrosis Factor-a (TNFa) produced in E. coli.
| Tag | Tag Free |
| Expression Host | E. coli |
Purified recombinant protein of Human forkhead box O4 (FOXO4), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Purified recombinant protein of Staphylococcus aureus SaCas9 containing Simian Virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- terminal of the protein, with N-terminal His tag, expressed in E. coli
| Tag | N-His |
| Expression Host | E. coli |
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Proteins
- Recombinant Proteins
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Species
- Human (4463)
- Mouse (102)
- Rat (21)
- Escherichia coli (16)
- Blomia tropicalis (10)
- SARS-CoV-2 (8)
- Dermatophagoides pteronyssinus (6)
- Streptococcus Pyogenes (5)
- Staphylococcus aureus (4)
- Orf Virus (3)
- Bovine (2)
- Dermatophagoides farinae (2)
- Rabbit (2)
- Streptococcus sp. (2)
- Alternaria alternata (1)
- Betula pendula (1)
- E.coli (1)
- Enterobacteria phage T4 (1)
- Escherichia phage T7 (1)
- HIV (1)
- Hepatitis B Virus (1)
- Humulus japonicus (1)
- Lachnospiraceae bacterium ND2006 (1)
- Lepidoglyphus destructor (1)
- Leptotrichia wadei (1)
- Macaca fascicularis (1)
- Mucor hiemalis (1)
- Mus musculus (1)
- Saccharomyces cerevisiae (1)
- Serratia marcescens (1)
- Staphylococcus aureus subsp. (1)
- Streptomyces avidinii (1)
- Streptomyces plicatus (1)
- Tobacco etch virus (1)
- Vaccinia virus (1)
Target Symbol
- VEGFA (27)
- TNF ALPHA (13)
- FGF2 (12)
- IL6 (12)
- IGF1 (10)
- IL33 (10)
- PTPRN (9)
- BDNF (8)
- EOTAXIN 3 (8)
- FGF1 (8)
- IL1 BETA (8)
- IL4 (8)
- NFYA (8)
- NRG1 (8)
- ALPHA SYNUCLEIN (7)
- BMP2 (7)
- BNP (7)
- CSF2 (7)
- EGF (7)
- FABP4 (7)
- HMG1 (7)
- LIF (7)
- SDF1 (7)
- CAMK2B (6)
- FERRITIN HEAVY CHAIN (6)
- FSTL1 (6)
- GABARAP (6)
- HIP2 (6)
- IL13 (6)
- IL1B (6)
- IL22 (6)
- IL3 (6)
- IL8 (6)
- INTERFERON ALPHA 2 (6)
- MIF (6)
- PDGF AA (6)
- SONIC HEDGEHOG (6)
- SUMO 3 (6)
- TGIF (6)
- TNF (6)
- TRAIL (6)
- TRIM72 (6)
- APOLIPOPROTEIN E (5)
- BIRC5 (5)
- BLNK (5)
- CNTF (5)
- DESMIN (5)
- GDNF (5)
- GLUTAMINE SYNTHETASE (5)
- IL15 (5)
- MAP1LC3A (5)
- NEUROTROPHIN 3 (5)
- NHP2 (5)
- PDGFA (5)
- S100A8 (5)
- TXN (5)
- ADIPOQ (4)
- AMPHIREGULIN (4)
- ANNEXIN V (4)
- ANNEXIN VI (4)
- APE1 (4)
- ASB13 (4)
- ASF1A (4)
- ASS1 (4)
- BETA ARRESTIN 1 (4)
- BID (4)
- BMP4 (4)
- CALCITONIN (4)
- CALRETICULIN 3 (4)
- CASPASE 10 (4)
- CSNK1G2 (4)
- CSPS (4)
- CXCL14 (4)
- CYSTATIN A (4)
- EGR1 (4)
- EIF1A (4)
- EIF4E (4)
- EIF4EBP1 (4)
- FGF21 (4)
- FIBRONECTIN (4)
- GABARAPL1 (4)
- GADD45B (4)
- GLUTATHIONE TRANSFERASE ZETA 1 (4)
- HAO1 (4)
- HIST1H2AK (4)
- IL17 (4)
- IL17F (4)
- IL1RA (4)
- IL2 (4)
- IL7 (4)
- INHIBIN BETA C CHAIN (4)
- INTERFERON GAMMA (4)
- KGF (4)
- KLF6 (4)
- KPNA2 (4)
- LACTB2 (4)
- LAP2 (4)
- LEPTIN (4)
- LSM4 (4)
- LYSOPHOSPHOLIPASE 1 (4)
Tags
- N-His (2604)
- Tag Free (511)
- N-GST and C-His (435)
- C-His (364)
- N-GST and C-HIS (150)
- N-GST (80)
- N-His-ABP (Albumin-Binding Protein) (52)
- His-tag (49)
- N-His&C-His (31)
- N-His-Trx (18)
- N-T7&C-His (18)
- N-Trx&His (18)
- N-GST, C-His (13)
- N-HIS (9)
- Tag-free (8)
- N-6His (6)
- N-GST&His (6)
- N-His&SUMO (6)
- N-His PDCD1(Pro21-Val170) (5)
- N-His-GST (4)
- C-Avi&6His (3)
- C-HIS (3)
- His-tagged, myc-tagged (3)
- N-Avi (3)
- N-His&C-Fc (3)
- N-His&T7 (3)
- N-MARI (3)
- N-Trx (3)
- Myc-DDK (2)
- N-His-PDCD1(Pro21-Val170) (2)
- N-terminal His (2)
- N-terminal His-PDCD1(Pro21-Val170) tag (2)
- C-DDK (1)
- C-DDK-His (1)
- C-HIS/C (1)
- C-Myc/DDK (1)
- DDK-tag (1)
- His tag (1)
- N-AVI (1)
- N-DDK (1)
- N-FUSION (1)
- N-GFP (1)
- N-GST(CKM) and N-His(CKB) (1)
- N-HIS and C-AVIPLUS (1)
- N-PROTEIN (1)
- N-T7 (1)
- N-TRX (1)
- N-TRXA (1)
- N-Trx-His (1)
- N-terminal His-tag (1)
Families
- Druggable Genome (2108)
- Secreted Protein (668)
- Transcription Factors (629)
- Transmembrane (607)
- ES Cell Differentiation/IPS (232)
- Protein Kinase (156)
- Stem cell - Pluripotency (154)
- Protease (122)
- Adult stem cells (80)
- Embryonic stem cells (68)
- Phosphatase (56)
- Induced pluripotent stem cells (38)
- Ion Channels: Other (35)
- Cancer stem cells (30)
- Stem cell relevant signaling - TGFb/BMP signaling pathway (25)
- Stem cell relevant signaling - JAK/STAT signaling pathway (22)
- Stem cell relevant signaling - Wnt Signaling pathway (15)
- Stem cell relevant signaling - DSL/Notch pathway (13)
- 10 mM Tris-HCl (12)
- GPCR (12)
- Nuclear Hormone Receptor (12)
- the solution can be further dialyzed against PBS (phosphate buffered saline (12)
- 2 mM dithiothreitol (10)
-
ELISA.
Western Blot. (10) - e.g. Dulbecco's PBS). (10)
- Ion Channels: Potassium (8)
- both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (8)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml (6) - P450 (6)
- pH 7.4). For immunization purposes (6)
- Ion Channels: Transient receptor potential (4)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml (4) -
pH 7.4).
For immunization purposes (4) -
pH 7.4).
For immunization purposes (4) - Ion Channels: Cys-loop Receptors (3)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies. (3) - Western Blot. (3)
- 0.1% (w/v) insulin (2)
- 10mM Tris-HCl (2)
- 1mM DTT (2)
- 2 mM dithiothreitol (2)
-
2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes (2) - 2mM dithiothreitol (2)
- 63mM sodium phosphate (2)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above) (2) - Can be used as standard in a Sandwich ELISA. (2)
- Ion Channels: Sodium (2)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above) (2) -
Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris (2) -
both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (2) - e.g. Dulbeccos PBS). (2)
- e.g.Dulbecco's PBS). (2)
- protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl (2)
- protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl (2)
- the solution can be further dialyzed against PBS (Phosphate Buffered Saline (2)
-
ELISA.
Western blotting. (1) -
Positive control for Western blot analysis.
Standard for ELISA. (1) -
Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T (1) -
Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A (1) - ELISA (1)
-
ELISA.
Western blotting. (1) - Forssmann WG (1)
- Hawkins JA (1)
- Hidaka Y (1)
- Ion Channels: ATP Receptors (1)
- Marx UC (1)
-
Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA (1) - Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21. (1)
- Neudecker P (1)
- Nourse A (1)
- Rosch P (1)
- Schulz A (1)
- Shimonishi Y (1)
-
Western Blot.
Protocol: 1. Lauber T (1) - Western Blotting. (1)
Pathways
- Metabolic pathways (317)
- Cytokine-cytokine receptor interaction (291)
- Pathways in cancer (269)
- MAPK signaling pathway (197)
- Jak-STAT signaling pathway (131)
- Hematopoietic cell lineage (110)
- Chemokine signaling pathway (107)
- Focal adhesion (107)
- Regulation of actin cytoskeleton (103)
- Melanoma (94)
- Toll-like receptor signaling pathway (87)
- Apoptosis (86)
- Natural killer cell mediated cytotoxicity (82)
- T cell receptor signaling pathway (82)
- NOD-like receptor signaling pathway (76)
- Alzheimer's disease (75)
- Systemic lupus erythematosus (68)
- Prostate cancer (67)
- Ubiquitin mediated proteolysis (65)
- TGF-beta signaling pathway (64)
- Fc epsilon RI signaling pathway (62)
- Neurotrophin signaling pathway (62)
- ErbB signaling pathway (61)
- Wnt signaling pathway (61)
- Cell cycle (60)
- Hypertrophic cardiomyopathy (HCM) (60)
- Cytosolic DNA-sensing pathway (58)
- Type I diabetes mellitus (57)
- Huntington's disease (56)
- Glioma (54)
- Graft-versus-host disease (54)
- Parkinson's disease (53)
- Oocyte meiosis (52)
- p53 signaling pathway (52)
- Dilated cardiomyopathy (51)
- RIG-I-like receptor signaling pathway (50)
- Amyotrophic lateral sclerosis (ALS) (49)
- Renal cell carcinoma (48)
- Melanogenesis (46)
- Pancreatic cancer (46)
- Insulin signaling pathway (45)
- Prion diseases (45)
- Acute myeloid leukemia (44)
- Adipocytokine signaling pathway (43)
- Colorectal cancer (43)
- Hedgehog signaling pathway (43)
- Small cell lung cancer (42)
- mTOR signaling pathway (42)
- Asthma (41)
- Pyrimidine metabolism (41)
- Chronic myeloid leukemia (40)
- PPAR signaling pathway (40)
- Endocytosis (39)
- Gap junction (39)
- Allograft rejection (38)
- Bladder cancer (38)
- B cell receptor signaling pathway (37)
- Purine metabolism (36)
- Ribosome (36)
- Basal cell carcinoma (35)
- Progesterone-mediated oocyte maturation (35)
- Base excision repair (33)
- Leukocyte transendothelial migration (33)
- Regulation of autophagy (33)
- Arginine and proline metabolism (32)
- Calcium signaling pathway (32)
- Nitrogen metabolism (32)
- Antigen processing and presentation (31)
- Type II diabetes mellitus (31)
- VEGF signaling pathway (31)
- GnRH signaling pathway (30)
- Axon guidance (29)
- Glycolysis / Gluconeogenesis (29)
- Autoimmune thyroid disease (28)
- Long-term potentiation (28)
- Spliceosome (28)
- Adherens junction (27)
- Epithelial cell signaling in Helicobacter pylori infection (27)
- Endometrial cancer (26)
- Vascular smooth muscle contraction (26)
- Oxidative phosphorylation (24)
- Cell adhesion molecules (CAMs) (22)
- Complement and coagulation cascades (22)
- Long-term depression (22)
- Neuroactive ligand-receptor interaction (22)
- Tyrosine metabolism (22)
- ECM-receptor interaction (21)
- Non-small cell lung cancer (20)
- Phenylalanine metabolism (20)
- Amino sugar and nucleotide sugar metabolism (19)
- Drug metabolism - other enzymes (19)
- Fc gamma R-mediated phagocytosis (19)
- Fructose and mannose metabolism (19)
- Glutathione metabolism (19)
- Porphyrin and chlorophyll metabolism (19)
- Primary immunodeficiency (19)
- Glycine (18)
- Metabolism of xenobiotics by cytochrome P450 (18)
- Tryptophan metabolism (18)
- serine and threonine metabolism (18)
- Arrhythmogenic right ventricular cardiomyopathy (ARVC) (17)
- Cysteine and methionine metabolism (17)
- Glycerophospholipid metabolism (17)
- SNARE interactions in vesicular transport (17)
- Alanine (16)
- Cardiac muscle contraction (16)
- Pathogenic Escherichia coli infection (16)
- RNA degradation (16)
- aspartate and glutamate metabolism (16)
- Sulfur metabolism (15)
- Mismatch repair (14)
- Nucleotide excision repair (14)
- Olfactory transduction (14)
- Pyruvate metabolism (14)
- Thyroid cancer (14)
- Tight junction (14)
- ABC transporters (13)
- Viral myocarditis (13)
- Homologous recombination (12)
- Inositol phosphate metabolism (12)
- Retinol metabolism (12)
- Aminoacyl-tRNA biosynthesis (11)
- DNA replication (11)
- Dorso-ventral axis formation (11)
- Drug metabolism - cytochrome P450 (11)
- Glyoxylate and dicarboxylate metabolism (11)
- Notch signaling pathway (11)
- Phosphatidylinositol signaling system (11)
- Terpenoid backbone biosynthesis (11)
- Androgen and estrogen metabolism (10)
- Arachidonic acid metabolism (10)
- Basal transcription factors (10)
- Citrate cycle (TCA cycle) (10)
- Propanoate metabolism (10)
- Butanoate metabolism (9)
- Fatty acid metabolism (9)
- Lysosome (9)
- Maturity onset diabetes of the young (9)
- Pantothenate and CoA biosynthesis (9)
- Valine (9)
- Ether lipid metabolism (8)
- Lysine degradation (8)
- Methane metabolism (8)
- Proteasome (8)
- beta-Alanine metabolism (8)
- leucine and isoleucine degradation (8)
- Galactose metabolism (7)
- N-Glycan biosynthesis (7)
- Nicotinate and nicotinamide metabolism (7)
- One carbon pool by folate (7)
- Pentose and glucuronate interconversions (7)
- Pentose phosphate pathway (7)
- Renin-angiotensin system (7)
- Selenoamino acid metabolism (7)
- Synthesis and degradation of ketone bodies (7)
- Ubiquinone and other terpenoid-quinone biosynthesis (7)
- Riboflavin metabolism (6)
- Vibrio cholerae infection (6)
- Glycosphingolipid biosynthesis - lacto and neolacto series (5)
- Heparan sulfate biosynthesis (5)
- Phenylalanine (5)
- Sphingolipid metabolism (5)
- Steroid biosynthesis (5)
- tyrosine and tryptophan biosynthesis (5)
- C21-Steroid hormone metabolism (4)
- Chondroitin sulfate biosynthesis (4)
- Histidine metabolism (4)
- Keratan sulfate biosynthesis (4)
- Linoleic acid metabolism (4)
- Non-homologous end-joining (4)
- O-Glycan biosynthesis (4)
- Primary bile acid biosynthesis (4)
- Starch and sucrose metabolism (4)
- Taste transduction (4)
- alpha-Linolenic acid metabolism (4)
- Circadian rhythm - mammal (3)
- D-Arginine and D-ornithine metabolism (3)
- Folate biosynthesis (3)
- RNA polymerase (3)
- Taurine and hypotaurine metabolism (3)
- Caffeine metabolism (2)
- D-Glutamine and D-glutamate metabolism (2)
- Glycosaminoglycan degradation (2)
- Glycosylphosphatidylinositol(GPI)-anchor biosynthesis (2)
- Limonene and pinene degradation (2)
- Other glycan degradation (2)
- leucine and isoleucine biosynthesis (2)
- Ascorbate and aldarate metabolism (1)
- Biosynthesis of unsaturated fatty acids (1)
- Cyanoamino acid metabolism (1)
- Fatty acid elongation in mitochondria (1)
- Glycerolipid metabolism (1)
- Glycosphingolipid biosynthesis - ganglio series (1)
- Lipoic acid metabolism (1)
- Protein export (1)
