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PPP1CA (1-330, His-tag) human recombinant protein, 10 µg
| Tag | His-tag |
| Expression Host | E. coli |
Products
PPP1CA (1-330, His-tag) human recombinant protein, 50 µg
| Tag | His-tag |
| Expression Host | E. coli |
MAPK3 / ERK1 (1-379, His-tag) human recombinant protein, 0.5 mg
| Tag | His-tag |
| Expression Host | E. coli |
ACTA2 / aortic smooth muscle Actin (3-377, His-tag) human recombinant protein, 50 µg
| Tag | His-tag |
| Expression Host | E. coli |
RAMP3 (24-118, His-tag) human recombinant protein, 20 µg
| Tag | His-tag |
| Expression Host | E. coli |
PRKACB (1-398, His-tag) human recombinant protein, 0.1 mg
| Tag | His-tag |
| Expression Host | E. coli |
RAMP3 (24-118, His-tag) human recombinant protein, 0.1 mg
| Tag | His-tag |
| Expression Host | E. coli |
B-Raf proto-oncogene (432-766, His-tag) human recombinant protein, 0.1 mg
| Tag | His-tag |
| Expression Host | E. coli |
B-Raf proto-oncogene (432-766, His-tag) human recombinant protein, 0.5 mg
| Tag | His-tag |
| Expression Host | E. coli |
PRKACB (1-398, His-tag) human recombinant protein, 0.5 mg
| Tag | His-tag |
| Expression Host | E. coli |
G protein alpha Q / GNAQ (1-359, His-tag) human recombinant protein, 0.5 mg
| Tag | His-tag |
| Expression Host | E. coli |
G protein alpha Q / GNAQ (1-359, His-tag) human recombinant protein, 0.1 mg
| Tag | His-tag |
| Expression Host | E. coli |
RAMP1 (27-117, His-tag) human recombinant protein, 0.25 mg
| Tag | His-tag |
| Expression Host | E. coli |
RAMP1 (27-117, His-tag) human recombinant protein, 50 µg
| Tag | His-tag |
| Expression Host | E. coli |
KCNMB3 (82-207, His-tag) human recombinant protein, 0.1 mg
| Tag | His-tag |
| Expression Host | E. coli |
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Category
Species
Target Symbol
Families
- Druggable Genome (27)
- Protein Kinase (10)
- Transmembrane (7)
- ES Cell Differentiation/IPS (2)
- Ion Channels: Other (2)
-
10mM pNPP.
2. Pre-warm reaction mix in heat block at 37°C.
3. Add recombinant PPP1CA solution with various concentrations (2ug (1) - 1mM MnCl2 (1)
- 2mM DTT (1)
-
3ug) in 200ul reaction buffer.
4. Mix by inversion and incubate at 37°C for 10 minutes.
5. Stop the reaction by adding the 800ul of 0.1N NaOH.
6. Record the absorbance at 405nm. (1) - 50mM NaCl (1)
- Phosphatase (1)
-
Protocol: Activity Assay
1. Prepare a 200 ul reaction mix into a suitable container : The final concentrations are 10mM Tris (pH 7.5) (1) - Secreted Protein (1)
