Products

Proteins

Recombinant Proteins

Human

TNF ALPHA

IGF1

FGF1

IL1 BETA

NRG1

VEGFA

BDNF

EOTAXIN 3

IL4

IL6

SDF1

BMP2

FSTL1

IL33

IL8

INTERFERON ALPHA 2

SONIC HEDGEHOG

BNP

IL3

GDNF

IL2

NEUROTROPHIN 3

APOLIPOPROTEIN E

CALCITONIN

CXCL14

FGF2

FGF21

FIBRONECTIN

IL15

IL17

IL17F

IL1RA

IL7

INHIBIN BETA C CHAIN

INTERFERON GAMMA

KGF

LEPTIN

LIF

NGAL

ONCOSTATIN M

PARATHYROID HORMONE

14-3-3 SIGMA

ANGIOGENIN

APOLIPOPROTEIN A I

APOLIPOPROTEIN CII

BD2

BETA CASEIN

BETA DEFENSIN 1

BMP4

C8

CARDIOTROPHIN 1

CCL28

CCL3

CD40L

CGRP

CXCL5

CXCL7

EOTAXIN

FGF12

FGF17

FGF19

FGF9

G CSF

GALECTIN 3

GM CSF

GRO BETA

GRO GAMMA

HAPTOGLOBIN

HEPC

HTN3

I 309

IL1 ALPHA

IL16

IL1F10

IL20

IL22

IL31

IL37

ISG15

LEC

LTA

MACROPHAGE INFLAMMATORY PROTEIN 3

MACROPHAGE INFLAMMATORY PROTEIN 4

MCP2

MELANOMA INHIBITORY ACTIVITY

NEUROKININ B

NGF

RANTES

RBP4

RESISTIN

RNASE9

SCGB3A2

SERPINA12

SF20

TAC1

TAFA4

UCMA

CANT1

CD8A

CSF2

HEK293T

HEK293

E. coli

CHO

Insect

Sf9

Pichia

Baculovirus

Tag Free

N-His

C-His

N-GST and C-His

N-GST and C-HIS

N-GST

N-His-Trx

His-tag

N-His-ABP (Albumin-Binding Protein)

Tag-free

N-Avi

N-His-PDCD1(Pro21-Val170)

C-HIS

N-HIS

N-T7

N-Trx-His

N-terminal His

N-terminal His-PDCD1(Pro21-Val170) tag

Druggable Genome

Secreted Protein

Transmembrane

Transcription Factors

ES Cell Differentiation/IPS

Stem cell - Pluripotency

Protein Kinase

Protease

Adult stem cells

Embryonic stem cells

Phosphatase

Induced pluripotent stem cells

Ion Channels: Other

Cancer stem cells

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Stem cell relevant signaling - Wnt Signaling pathway

10 mM Tris-HCl

GPCR

Nuclear Hormone Receptor

the solution can be further dialyzed against PBS (phosphate buffered saline

2 mM dithiothreitol

ELISA.
Western Blot.

e.g. Dulbecco's PBS).

Ion Channels: Potassium

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

P450

pH 7.4). For immunization purposes

Ion Channels: Transient receptor potential

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4).
For immunization purposes

pH 7.4).
For immunization purposes

Ion Channels: Cys-loop Receptors

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Can be used as standard in a Sandwich ELISA.

Ion Channels: Sodium

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).

e.g.Dulbecco's PBS).

including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

ELISA.
Western blotting.

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA

ELISA.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Ion Channels: ATP Receptors

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

Cytokine-cytokine receptor interaction

Pathways in cancer

MAPK signaling pathway

Jak-STAT signaling pathway

Hematopoietic cell lineage

Chemokine signaling pathway

NOD-like receptor signaling pathway

Melanoma

Toll-like receptor signaling pathway

Regulation of actin cytoskeleton

Graft-versus-host disease

T cell receptor signaling pathway

Type I diabetes mellitus

Cytosolic DNA-sensing pathway

Apoptosis

TGF-beta signaling pathway

Asthma

Allograft rejection

Fc epsilon RI signaling pathway

Natural killer cell mediated cytotoxicity

Hypertrophic cardiomyopathy (HCM)

RIG-I-like receptor signaling pathway

Focal adhesion

Prion diseases

Alzheimer's disease

Systemic lupus erythematosus

Autoimmune thyroid disease

Basal cell carcinoma

Hedgehog signaling pathway

Dilated cardiomyopathy

mTOR signaling pathway

Adipocytokine signaling pathway

Bladder cancer

Complement and coagulation cascades

p53 signaling pathway

Melanogenesis

Neurotrophin signaling pathway

Amyotrophic lateral sclerosis (ALS)

Antigen processing and presentation

Long-term depression

Type II diabetes mellitus

Prostate cancer

Regulation of autophagy

Wnt signaling pathway

Epithelial cell signaling in Helicobacter pylori infection

Glioma

Oocyte meiosis

Progesterone-mediated oocyte maturation

ECM-receptor interaction

ErbB signaling pathway

Neuroactive ligand-receptor interaction

Pancreatic cancer

Renal cell carcinoma

VEGF signaling pathway

Metabolic pathways

Small cell lung cancer

Axon guidance

Huntington's disease

Leukocyte transendothelial migration

PPAR signaling pathway

Acute myeloid leukemia

Endocytosis

Cell adhesion molecules (CAMs)

Primary immunodeficiency

Proteasome

Cell cycle

GnRH signaling pathway

Viral myocarditis

Arachidonic acid metabolism

Ether lipid metabolism

N-Glycan biosynthesis

Purine metabolism

Pyrimidine metabolism

Renin-angiotensin system

Glycerophospholipid metabolism

Glycosaminoglycan degradation

Linoleic acid metabolism

Vascular smooth muscle contraction

alpha-Linolenic acid metabolism

Amino sugar and nucleotide sugar metabolism

Fc gamma R-mediated phagocytosis

Gap junction

Glutathione metabolism

Starch and sucrose metabolism

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1

Yes

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