Products

Proteins

Recombinant Proteins

Human

BDNF

BMP2

EGF

FGF21

KGF

BMP4

FGF19

HBZ

C-MYC

FGF10

OCT4

BXDC2

CCN4

CD117

CD34

CDX2

CRIPTO1

DPPA2

EOMES

FGF4

GATA6

GLI2

KLF5

NODAL

OTX2

PAX3

PAX4

PAX6

PAX7

PDX1

PROX1

REX1

SMAD3

TAT

ZIC3

HEK293T

E. coli

HEK293

CHO

Sf9

N-His

Tag Free

N-GST and C-His

N-GST and C-HIS

N-T7

Druggable Genome

Secreted Protein

Transmembrane

Transcription Factors

ES Cell Differentiation/IPS

Stem cell - Pluripotency

Protein Kinase

Protease

Adult stem cells

Embryonic stem cells

Phosphatase

Induced pluripotent stem cells

Ion Channels: Other

Cancer stem cells

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Stem cell relevant signaling - Wnt Signaling pathway

10 mM Tris-HCl

GPCR

Nuclear Hormone Receptor

the solution can be further dialyzed against PBS (phosphate buffered saline

2 mM dithiothreitol

ELISA.
Western Blot.

e.g. Dulbecco's PBS).

Ion Channels: Potassium

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

P450

pH 7.4). For immunization purposes

Ion Channels: Transient receptor potential

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4).
For immunization purposes

pH 7.4).
For immunization purposes

Ion Channels: Cys-loop Receptors

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Can be used as standard in a Sandwich ELISA.

Ion Channels: Sodium

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).

e.g.Dulbecco's PBS).

including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

ELISA.
Western blotting.

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA

ELISA.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Ion Channels: ATP Receptors

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

Pathways in cancer

MAPK signaling pathway

Melanoma

Regulation of actin cytoskeleton

TGF-beta signaling pathway

Cytokine-cytokine receptor interaction

Endocytosis

Basal cell carcinoma

Hedgehog signaling pathway

Acute myeloid leukemia

Hematopoietic cell lineage

Huntington's disease

Melanogenesis

Neurotrophin signaling pathway

Bladder cancer

Endometrial cancer

ErbB signaling pathway

Pancreatic cancer

Focal adhesion

Gap junction

Glioma

Non-small cell lung cancer

Prostate cancer

Cell cycle

Chronic myeloid leukemia

Colorectal cancer

Wnt signaling pathway

Jak-STAT signaling pathway

Maturity onset diabetes of the young

Small cell lung cancer

Thyroid cancer

Adherens junction

Cell adhesion molecules (CAMs)

Cysteine and methionine metabolism

Metabolic pathways

Phenylalanine

Phenylalanine metabolism

Type II diabetes mellitus

Tyrosine metabolism

Ubiquinone and other terpenoid-quinone biosynthesis

tyrosine and tryptophan biosynthesis

1

2

Yes

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