Products

Proteins

Recombinant Proteins

Human

BDNF

BMP2

APOLIPOPROTEIN E

EGF

FGF21

KGF

LIF

BMP4

CD72

FGF19

CD8A

GATA3

FGF10

NOTCH1

OCT4

WNT6

CCN4

CD117

CD34

CDX2

DKK1

FGF4

GATA1

GLI2

HES1

LEF1

PAX3

PAX6

PAX7

REX1

SFRP4

SFRP5

WNT1

WNT2

WNT3A

HEK293T

E. coli

HEK293

Sf9

CHO

Insect

N-His

Tag Free

N-GST and C-HIS

N-Trx&His

N-GST and C-His

N-His-ABP (Albumin-Binding Protein)

N-T7

Tag-free

Druggable Genome

Secreted Protein

Transmembrane

Transcription Factors

ES Cell Differentiation/IPS

Stem cell - Pluripotency

Protein Kinase

Protease

Adult stem cells

Embryonic stem cells

Phosphatase

Induced pluripotent stem cells

Ion Channels: Other

Cancer stem cells

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Stem cell relevant signaling - Wnt Signaling pathway

10 mM Tris-HCl

GPCR

Nuclear Hormone Receptor

the solution can be further dialyzed against PBS (phosphate buffered saline

2 mM dithiothreitol

ELISA.
Western Blot.

e.g. Dulbecco's PBS).

Ion Channels: Potassium

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

P450

pH 7.4). For immunization purposes

Ion Channels: Transient receptor potential

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4).
For immunization purposes

pH 7.4).
For immunization purposes

Ion Channels: Cys-loop Receptors

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Can be used as standard in a Sandwich ELISA.

Ion Channels: Sodium

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).

e.g.Dulbecco's PBS).

including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

ELISA.
Western blotting.

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA

ELISA.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Ion Channels: ATP Receptors

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

Pathways in cancer

MAPK signaling pathway

Melanoma

Regulation of actin cytoskeleton

Basal cell carcinoma

Cytokine-cytokine receptor interaction

Hedgehog signaling pathway

Melanogenesis

Endocytosis

Hematopoietic cell lineage

TGF-beta signaling pathway

Acute myeloid leukemia

Huntington's disease

Neurotrophin signaling pathway

Wnt signaling pathway

Endometrial cancer

Prostate cancer

Alzheimer's disease

Bladder cancer

ErbB signaling pathway

Focal adhesion

Gap junction

Glioma

Jak-STAT signaling pathway

Non-small cell lung cancer

Pancreatic cancer

B cell receptor signaling pathway

Cell adhesion molecules (CAMs)

Antigen processing and presentation

Notch signaling pathway

Primary immunodeficiency

T cell receptor signaling pathway

Dorso-ventral axis formation

Maturity onset diabetes of the young

Prion diseases

Adherens junction

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Colorectal cancer

Thyroid cancer

1

2

Yes

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