Products

Human IgA (Secretory component, free and bound) sheep polyclonal antibody, HRP

Applications This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation HRP
TA355211 is a possible alternative to AP21476HR-N.

Guinea pig IgG (H+L chain), adsorbed goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/100,000-1/200,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Guinea Pig
Conjugation HRP

Bovine IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (1:1,000-10,000), ELISA (1:10,000 - 1:50,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1:500-1:2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Reactivities Bovine
Conjugation HRP

Goat Anti-Mouse IgG (H+L) HRP conjugated secondary antibody

Applications EIA and Western blots: 1:5,000-1:100,000. Immunohistochemistry: 1:500-1:5,000. Optimal working dilutions must be determined by end user.
Reactivities Mouse
Conjugation HRP

Goat Anti-Rabbit IgG (H+L) HRP conjugated secondary antibody

Applications EIA and Western blots: 1:5,000-1:100,000. Immunohistochemistry: 1:500-1:5,000. Optimal working dilutions must be determined by end user.
Reactivities Rabbit
Conjugation HRP

Goat Anti-Rabbit IgG (H+L) HRP conjugated secondary antibody

Applications EIA and Western blots: 1:5,000-1:100,000. Immunohistochemistry: 1:500-1:5,000. Optimal working dilutions must be determined by end user.
Reactivities Rabbit
Conjugation HRP

Goat Anti-Mouse IgG (H+L) HRP conjugated secondary antibody

Applications EIA and Western blots: 1:5,000-1:100,000. Immunohistochemistry: 1:500-1:5,000. Optimal working dilutions must be determined by end user.
Reactivities Mouse
Conjugation HRP

Human IgG2 (F(ab)2 specific) mouse monoclonal antibody, clone NI 6014 (HP 6014), HRP

Applications To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct Immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/25-1/100.
ELISA: from 1/250 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation HRP

Human IgD mouse monoclonal antibody, clone NI 158, HRP

Applications To identify the presence of IgD in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/500.
ELISA: from 1/1000 upwards.
Western blot: from 1/2000 upwards.
Reactivities Human
Conjugation HRP

Human IgA mouse monoclonal antibody, clone NI 69 (A89-034) and NI 184 (A89-035), HRP

Applications To identify the presence of IgA in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgA, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50.
ELISA: from 1/50 upwards.
Western blot: from 1/100 upwards.
Reactivities Human
Conjugation HRP

Human IgE mouse monoclonal antibody, clone NI 307, HRP

Applications To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/250.
ELISA: from 1/250 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation HRP

Human IgA2 mouse monoclonal antibody, clone NI 512 (A89-038), HRP

Applications To identify the presence of IgA2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct Immunoperoxidase staining of cytoplasmic IgA2, and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/20-1/100
ELISA: from 1/50 upwards.
Western blot: from 1/100 upwards.
Reactivities Human
Conjugation HRP

Human IgA1 mouse monoclonal antibody, clone NI 69-11 (A89-036), HRP

Applications To identify the presence of IgA1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, indirect immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA1, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50
ELISA: from 1/50 upwards
Western blot: from 1/100 upwards
Reactivities Human
Conjugation HRP

Human IgA2 (A2m2 Allotype Specific) mouse monoclonal antibody, clone NI 194-3 (A89-040), HRP

Applications To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Immunoperoxidase staining of cytoplasmic IgA2(m)2, and Immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50
ELISA: from 1/60 upwards.
Western blot: from 1/20 upwards.
Reactivities Human
Conjugation HRP