Products

Human IgG (H+L chain), adsorbed rabbit polyclonal antibody, AP

Applications Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1:2,000-1:40,000.
Western Blot: 1: 500-1:2,000.
Immunohistochemistry: 1:200-1:1,000.
Reactivities Human
Conjugation AP

Human IgG (H+L chain) chicken polyclonal antibody, AP

Applications ELISA: 1/2,000 - 1/10,000.
Western Blot: 1/500 - 1/2,500.
Immunohistochemistry: 1/200 - 1/1,000.
Reactivities Human
Conjugation AP

Human IgM (Fc5 mu specific), F(ab)2 Fragment goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/7,500-1/30,000.
Western Blot: 1/1000-1/5000.
Immunohistochemistry: 1/200-1/1000.
Note: This product has been assayed against 1.0 µg of Human IgM in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/5,000 to 1/50,000 is suggested for this product.
Reactivities Human
Conjugation AP