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Cytokeratin 18 (KRT18) mouse monoclonal antibody, clone Ks18.04, Purified
| Applications | IHC, WB |
| Reactivities | Bovine, Canine, Hamster, Human, Mouse, Porcine, Rat, Sheep, Trout, Zebrafish |
Cytokeratin 18 (KRT18) mouse monoclonal antibody, clone DC-10, FITC
| Applications | FC, IF, IHC |
| Reactivities | Human |
Cytokeratin 18 human recombinant protein, 0.25 mg
| Expression Host | E. coli |
Cytokeratin 18 bovine protein, 0.25 mg
| Protein Source | Liver |
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- 10 mM Tris-HCl (2)
- 2 mM dithiothreitol (2)
- the solution can be further dialyzed against PBS (phosphate buffered saline (2)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml (1) -
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml (1) -
both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (1) - both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (1)
-
e.g. Dulbeccos PBS).
See References 2 and 3 for more details. (1) - e.g.Dulbecco's PBS). (1)
-
pH 7.4).
For immunization purposes (1) -
pH 7.4).
For Immunization purposes (1)
