Products

Proteins

Recombinant Proteins

Human

VIM

E. coli

Druggable Genome

Transmembrane

Transcription Factors

Secreted Protein

ES Cell Differentiation/IPS

Protein Kinase

WB

Stem cell - Pluripotency

Protease

GPCR

Adult stem cells

Embryonic stem cells

Nuclear Hormone Receptor

Cancer stem cells

Induced pluripotent stem cells

Phosphatase

Ion Channels: Other

Ion Channels: Potassium

Stem cell relevant signaling - JAK/STAT signaling pathway

P450

Stem cell relevant signaling - DSL/Notch pathway

Stem cell relevant signaling - Wnt Signaling pathway

Ion Channels: Transient receptor potential

Stem cell relevant signaling - TGFb/BMP signaling pathway

Ion Channels: Cys-loop Receptors

Ion Channels: Glutamate Receptors

Ion Channels: Sodium

Ion Channels: ATP Receptors

Ion Channels: Calcium

10 mM Tris-HCl

2 mM dithiothreitol

ELISA.
Western Blot.

Ion Channels: Cyclic nucleotide gated

the solution can be further dialyzed against PBS (phosphate buffered saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/5

10mM Tris-HCl

2mM dithiothreitol

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA: this protein is suitable for use as a Mouse IgE standard in ELISA assays.

Functional Assays.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Can be used as standard in a Sandwich ELISA.

ELISA

ELISA.
Western blotting.

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Immunofluorescence Microscopy (1/1

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Secreted

Specific methodologies have not been tested using this product.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbecco's PBS).

e.g. Dulbeccos PBS).
See References 2 and 3 for more details.

e.g.Dulbecco's PBS).

pH 7.4).
For immunization purposes

pH 7.4).
For Immunization purposes

pH 7.4).
For immunization purposes

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

2

Yes