AAVS1 donor vector with AAVS1 homologous arms cloned (Blasticidin selection), EF1a promoter, ready for exogenous gene cloning (10 ug)
Function | AAVS1 donor vector |
AAVS1 donor vector with AAVS1 homologous arms cloned (Blasticidin selection), EF1a promoter, ready for exogenous gene cloning (10 ug)
Function | AAVS1 donor vector |
Positive control for AAVS1 insertion (RFP insertion via CRISPR)
Function | AAVS1 donor vector |
ROSA26 donor vector with ROSA26 homologous arms cloned (Puro selection), ready for exogenous gene cloning (10 ug)
Function | ROSA26 donor vector |
pCas-Guide-GFP-CRISPRa vector, All-in-one Vector for CRISPR/Cas9 Gene activation with tGFP reporter, dCas9-VP64 and gRNA cloning site.
Function | All-in-one CRISPRa SAM vector |
ROSA26 donor vector with ROSA26 homologous arms, tGFP expression, and Puro selection (10 ug)
Function | ROSA26 donor vector |
AAVS1 donor vector with AAVS1 homologous arms, tGFP under EF1a promoter, and Puro selection (10 ug)
Function | AAVS1 donor vector |
Donor vector with AAVS1 homologous arms, C-terminal mGFP tag, Puro resistance, ready for transgene cloning
Function | AAVS1 donor vector |
pAAV-AC-Myc-DDK, AAV vector with C-terminal Myc-DDK tag
pLenti-N-tYFP, Lenti Vector with N-terminal tYFP tag, 10ug
N-Tag | tYFP |
E. coli Selection | Chloramphenicol (34 ug/mL) |
pLenti-Guide-Puro, gRNA cloning vector in lenti vector with puromycin as mammalian selection marker (10ug)
Function | gRNA only vectors |
pLenti-N-DDK-P2A-Puro, Lenti vector with N-terminal DDK tag with P2A-Puro
N-Tag | DDK |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Cell Selection | Puromycin |
pLenti-N-Myc-DDK-IRES-Puro, Lenti vector with N-terminal Myc-DDK tag and IRES-Puro, 10 ug
N-Tag | Myc-DDK |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Cell Selection | Puromycin |
A scrambled sequence in pCas-Guide-EF1a-GFP vector (10 µg)
Function | All-in-One CRISPR/Cas9 vectors |
HuSH shRNA GFP Retroviral Cloning Vector with blasticidin resistance (pGFP-B-RS Vector) 5 ug
Viral Packaging | Retroviral |
pCMV6-AC-mBFP, mammalian vector with C-terminal mBFP, 10ug
C-Tag | mBFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Cell Selection | Neomycin |