HuSH shRNA Retroviral Cloning Vector with blasticidin resistance (pB-RS Vector), 5 ug
Viral Packaging | Retroviral |
HuSH shRNA Retroviral Cloning Vector with blasticidin resistance (pB-RS Vector), 5 ug
Viral Packaging | Retroviral |
pCMV6-Entry2, mammalian vector with C-terminal Myc-DDK tag, 10ug
C-Tag | Myc-DDK |
E. coli Selection | Kanamycin (25 ug/mL) |
Cell Selection | Neomycin |
Vector kit to insert Cas9 at AAVS1 locus (puro selection), including AAVS1 gRNA vector (cat# GE100023), Cas9-Puro donor (cat# GE100037), and a scramble gRNA control (cat# GE100003)
Function | CRISPR/AAVS1 kit |
pLenti-E/hSyn-C-Myc-DDK-P2A-Puro, Lenti vector with CMV enhancer, Human synapsin I promoter, C-terminal Myc-DDK tag and P2A-Puro, 10 ug
C-Tag | Myc-DDK |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Cell Selection | Puromycin |
pCMV6-AN-Myc-DDK-AC-His, mammalian expression vector with N-terminal Myc-DDK tags and C-terminal His tag, 10 ug
C-Tag | His |
N-Tag | Myc-DDK |
E. coli Selection | Ampicillin (100 ug/mL) |
Cell Selection | Neomycin |
CRISPRa SAM vector kit containing pCas-Guide-CRISPRa (GE100055), pCRISPRa-Enhancer (GE100056) and pCas-Guide-CRISPRa-Scramble (GE100058)
Function | CRISPRa SAM vector kit |
HuSH shRNA RFP Lenti Cloning Vector (pRFP-C-shLenti Vector), 5 ug
Viral Packaging | Lentiviral |
pCas-Guide-Puro-CRISPRi-Scramble, scramble gRNA control in GE100083 vector (with Puro selection)
29-mer Scrambled shRNA Cassette in p-RFP-C-shLenti Vector, 5 ug
Viral Packaging | Lentiviral |
pLenti-Cas9-Myc-DDK-P2A-Puro control plasmid containing the expression cassette for Cas9-Myc-DDK fusion protein and Puromycin resistance.
Function | Cas9 only vectors |
29-mer Scrambled shRNA Cassette in pGFP-A-shAAV Vector, 5ug
Viral Packaging | Adeno-associated virus (AAV) |
CNY 9,500.00
2周
RFP negative transgenic cell lines (HEK293T cells containing RFP dead mutant at AAVS1 locus), can be edited to convert to RFP positive cells
Validated sgRNA targeting RFP dead mutant in GE100041C cells