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Human

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HIV

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Drosophila melanogaster

E.coli

Enterobacteria phage T4

Escherichia phage T7

Human herpesvirus

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Humon

Humulus japonicus

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Lachnospiraceae bacterium ND2006

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Mus musculus

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SIV

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Sheep

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Tobacco etch virus

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Vacciniavirus

Vibrio cholerae

viral

VEGFA

ALPHA 1 ANTITRYPSIN

NCALD

FGF1

NDRG2

S PROTEIN

IRF5

ISOCITRATE DEHYDROGENASE

ALPHA SYNUCLEIN

FGF2

RIBONUCLEASE INHIBITOR

CPNE1

FGFR1

IL6

MORTALITY FACTOR 4 LIKE 2

PD-L1

TNF ALPHA

UBE2D3

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M-CSF

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MAX

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BETA 2 MICROGLOBULIN

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CD3E

CD47

CTLA4

FHL1

HER2

HMG1

IL33

IL8

MBNL1

PCBP2

PCSK9

S100 CALCIUM BINDING PROTEIN A13

SULT1A1

TROPOMYOSIN 3

14-3-3 ZETA

ACRV1

ASPARTATE BETA HYDROXYLASE

CEACAM1

CSF2

ERBB 3

GLUTAMINE SYNTHETASE

GM CSF

HNF 4 ALPHA

HNRNP F

IL12B

IL1RA

MYELIN BASIC PROTEIN

NDUF3

P38

PGF

TIE1

ANNEXIN A2

ARSA

AURORA A

CARBONIC ANHYDRASE X

CD10

CD147

CD83

CUTA

DYNLL1

FHL2

FKBP2

GALE

ICAM1

IDH2

LAMP2

LIF

MICA

OSTEOPONTIN

PTPRN

ROR1

SIRP ALPHA

TAU

TIGIT

TNFRSF4

APOLIPOPROTEIN E

B RAF

BDNF

CARDIAC TROPONIN T

CD33

HEK293T

E. coli

HEK293

Sf9

Insect

CHO

Baculovirus

Pichia

Pichia pastoris

Mouse

Other

S. cerevisiae

Sf9 insect

H. vulgare

Human

Yeast

C-Myc/DDK

C-MYC/DDK

N-His

C-His

Tag Free

N-GST and C-His

C-Fc

C-Human Fc

C-6His

C-DDK

C-6×His

N-GST and C-HIS

N-GST

C-DDK/His

His-tag

Myc-DDK

C-Fc&His

C-Mouse Fc

N-His-ABP (Albumin-Binding Protein)

N-Human Fc

C-HIS

C-mFc

His-Tag

N-His&C-His

N-6His

C-6His-Avi

C-Mouse Fc and 6×His

C-8His

N-T7&C-His

N-Trx&His

C-hFc

C-Avi-6His

N-His-Trx

C-Fc-Avi

C-Human Fc and 6×His

C-10His

N-Fc

N-GST, C-His

hIgG-His-Tag

N-HIS

N-His&SUMO

C- 6×His

N-6×His

N-GST&His

Tag-free

C-Avi&6His

C-6xHis

hIgG-Tag

C-Fc&AVI

N-Avi

N-His PDCD1(Pro21-Val170)

N-His/DDK

N-Mouse Fc and C-6×His

C-6×His tag and C-Human Fc

C-AVI&His

C-mouse Fc

N-6His-Avi

N-8His

N-His&C-Fc

N-His&T7

N-His-GST

N-MARI

N-Trx

C-Fc/His

C-Flag

C-hFc&His

His-tagged, myc-tagged

N-DDK

N-mFc

C-3×Flag Tag

C-6xHis/AVI

C-7×His

C-DDK-His

C-FC

C-Fc-Flag&C-Fc-6His

C-HUMAN

N-6His-Flag

N-8His&N-FLAG

N-FC

N-Fc-FLAG

N-His&Flag

N-His-PDCD1(Pro21-Val170)

N-Mouse Fc

N-terminal His

N-terminal His-PDCD1(Pro21-Val170) tag

C-AVI

C-AVI-PLUS

C-Avi

C-Avi-10His

C-Avi-His

C-DDK/10xHis

C-DDK/6xHis

C-FC-6HIS&C-FC-Flag

C-FC/6xHis

C-FLAG-Fc

C-Fc Human

C-Fc Mouse

C-Flag,C-6His

C-GST

C-HIS/C

C-HIS/DDK

C-His&AVI

C-His&Flag

C-His&HA&AVI

C-His/AVIplus

C-His/DDK

C-Human Fc, Avi

C-Human Fc-Avi

C-MIgG2a

C-hIgG1 Fc

C-hIgG2 Fc

C-hIgG4 Fc

C-hIgG4P Fc

C-mFC

C-mFc&His

C-mIgG2AFc

C-mIgG2a Fc

C-mIgG2aFc

C-terminal DDK

C-terminal His

DDK-tag

His tag

N-10*His

N-10His

N-10His-Flag

N-6His-FLAG

N-8His-Flag

N-AVI

N-AVI&His

N-Avi&6His

N-DDK/His

N-FLAG

N-FUSION

N-Flag&C-His

N-Flag&His

N-GFP

N-GST(CKM) and N-His(CKB)

N-GST/C-His

N-HIS and C-AVIPLUS

N-His&MBP

N-PROTEIN

N-T7

N-TRX

N-TRXA

N-Trx-His

N-hFc

N-terminal His-tag

N-terminal murine Fc Tag

No

Tag free

PE

FITC

HRP

Yes

MVPro

SDS PAGE

Druggable Genome

Transmembrane

Secreted Protein

Transcription Factors

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

Phosphatase

Adult stem cells

Ion Channels: Other

Embryonic stem cells

Nuclear Hormone Receptor

P450

Cancer stem cells

GPCR

Induced pluripotent stem cells

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Stem cell relevant signaling - TGFb/BMP signaling pathway

ELISA

Stem cell relevant signaling - Wnt Signaling pathway

SDS-PAGE

Ion Channels: Potassium

Bioactivity

Ion Channels: Glutamate Receptors

the solution can be further dialyzed against PBS (phosphate buffered saline

Ion Channels: ATP Receptors

Ion Channels: Cys-loop Receptors

10 mM Tris-HCl

2 mM dithiothreitol

Enzyme Activity

e.g. Dulbecco's PBS).

Flow Cyt

ELISA.
Western Blot.

Ion Channels: Transient receptor potential

Ion Channels: Sodium

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4).
For immunization purposes

pH 7.4).
For immunization purposes

Ion Channels: Cyclic nucleotide gated

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Can be used as standard in a Sandwich ELISA.

ELISA.

FACS

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/40

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western blot: 1/10

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Western blotting.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA.
Functional Assays.

ELISA.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

Metabolic pathways

Cytokine-cytokine receptor interaction

Pathways in cancer

MAPK signaling pathway

Focal adhesion

Regulation of actin cytoskeleton

Jak-STAT signaling pathway

Chemokine signaling pathway

Endocytosis

Natural killer cell mediated cytotoxicity

Hematopoietic cell lineage

Neurotrophin signaling pathway

Cell adhesion molecules (CAMs)

T cell receptor signaling pathway

Toll-like receptor signaling pathway

Ubiquitin mediated proteolysis

Insulin signaling pathway

Purine metabolism

Alzheimer's disease

Axon guidance

ErbB signaling pathway

Cell cycle

Wnt signaling pathway

Leukocyte transendothelial migration

Apoptosis

Prostate cancer

Pancreatic cancer

Melanoma

Huntington's disease

Fc epsilon RI signaling pathway

Tight junction

Progesterone-mediated oocyte maturation

Adherens junction

Calcium signaling pathway

Colorectal cancer

GnRH signaling pathway

Lysosome

Oocyte meiosis

TGF-beta signaling pathway

Hypertrophic cardiomyopathy (HCM)

RIG-I-like receptor signaling pathway

Renal cell carcinoma

Dilated cardiomyopathy

Pyrimidine metabolism

NOD-like receptor signaling pathway

Chronic myeloid leukemia

Fc gamma R-mediated phagocytosis

Spliceosome

B cell receptor signaling pathway

Glioma

Systemic lupus erythematosus

VEGF signaling pathway

PPAR signaling pathway

Epithelial cell signaling in Helicobacter pylori infection

Small cell lung cancer

Adipocytokine signaling pathway

Antigen processing and presentation

Parkinson's disease

Glycolysis / Gluconeogenesis

Bladder cancer

Complement and coagulation cascades

p53 signaling pathway

Gap junction

Cytosolic DNA-sensing pathway

Acute myeloid leukemia

Melanogenesis

Vascular smooth muscle contraction

Endometrial cancer

Non-small cell lung cancer

Phosphatidylinositol signaling system

Pathogenic Escherichia coli infection

Long-term potentiation

Autoimmune thyroid disease

Type I diabetes mellitus

Arginine and proline metabolism

Oxidative phosphorylation

Long-term depression

Amyotrophic lateral sclerosis (ALS)

mTOR signaling pathway

ECM-receptor interaction

Viral myocarditis

Metabolism of xenobiotics by cytochrome P450

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Drug metabolism - cytochrome P450

Amino sugar and nucleotide sugar metabolism

Inositol phosphate metabolism

Allograft rejection

Glutathione metabolism

Type II diabetes mellitus

Cardiac muscle contraction

Graft-versus-host disease

Neuroactive ligand-receptor interaction

Glycerophospholipid metabolism

Tyrosine metabolism

Thyroid cancer

Drug metabolism - other enzymes

Fructose and mannose metabolism

Retinol metabolism

Prion diseases

Valine

Vibrio cholerae infection

Pyruvate metabolism

Starch and sucrose metabolism

Regulation of autophagy

Alanine

Tryptophan metabolism

aspartate and glutamate metabolism

Cysteine and methionine metabolism

Primary immunodeficiency

Proteasome

RNA degradation

leucine and isoleucine degradation

Arachidonic acid metabolism

Glycerolipid metabolism

Ribosome

Fatty acid metabolism

Nitrogen metabolism

Androgen and estrogen metabolism

Hedgehog signaling pathway

Porphyrin and chlorophyll metabolism

Base excision repair

Nucleotide excision repair

Asthma

Citrate cycle (TCA cycle)

Pentose phosphate pathway

Galactose metabolism

Butanoate metabolism

Propanoate metabolism

Sphingolipid metabolism

Histidine metabolism

Basal cell carcinoma

Lysine degradation

DNA replication

Notch signaling pathway

Olfactory transduction

beta-Alanine metabolism

Aminoacyl-tRNA biosynthesis

Nicotinate and nicotinamide metabolism

Glycine

serine and threonine metabolism

SNARE interactions in vesicular transport

Phenylalanine metabolism

Dorso-ventral axis formation

Ether lipid metabolism

Maturity onset diabetes of the young

Selenoamino acid metabolism

Renin-angiotensin system

Sulfur metabolism

Linoleic acid metabolism

Mismatch repair

Glyoxylate and dicarboxylate metabolism

Pantothenate and CoA biosynthesis

Pentose and glucuronate interconversions

Riboflavin metabolism

Glycosaminoglycan degradation

RNA polymerase

Other glycan degradation

Homologous recombination

Ascorbate and aldarate metabolism

N-Glycan biosynthesis

Terpenoid backbone biosynthesis

Glycosphingolipid biosynthesis - lacto and neolacto series

Basal transcription factors

One carbon pool by folate

Primary bile acid biosynthesis

Taste transduction

alpha-Linolenic acid metabolism

C21-Steroid hormone metabolism

Folate biosynthesis

O-Glycan biosynthesis

Glycosphingolipid biosynthesis - globo series

Limonene and pinene degradation

Keratan sulfate biosynthesis

Synthesis and degradation of ketone bodies

Thiamine metabolism

Heparan sulfate biosynthesis

ABC transporters

Biosynthesis of unsaturated fatty acids

Circadian rhythm - mammal

Methane metabolism

Steroid biosynthesis

Non-homologous end-joining

Glycosylphosphatidylinositol(GPI)-anchor biosynthesis

Chondroitin sulfate biosynthesis

Taurine and hypotaurine metabolism

Ubiquinone and other terpenoid-quinone biosynthesis

Glycosphingolipid biosynthesis - ganglio series

Phenylalanine

leucine and isoleucine biosynthesis

tyrosine and tryptophan biosynthesis

Caffeine metabolism

Fatty acid elongation in mitochondria

Protein export

Fatty acid biosynthesis

Lysine biosynthesis

Vitamin B6 metabolism

D-Glutamine and D-glutamate metabolism

Cyanoamino acid metabolism

Biotin metabolism

D-Arginine and D-ornithine metabolism

O-Mannosyl glycan biosynthesis

Lipoic acid metabolism

Human

E. coli

Human 293

P.Pichia

Baculovirus

HEK293

293F

Insect

CHO Stable

Expi293

Mammalian

Pichia pastoris

1

2

3

Yes

In Stock