Proteins

Proteins

Recombinant Proteins

Human

BDNF

BMP2

EGF

BMP4

HBZ

FGF21

KGF

FGF10

FGF19

KLF4

NANOG

POU5F1

C-MYC

CD34

SMAD3

BMP5

BMP6

BMP7

BMP8B

CD14

GDF5

OCT4

SMAD2

SOX2

BXDC2

CCN4

CD117

CDX2

CRIPTO1

DPPA2

EOMES

FGF4

GATA6

GLI2

KLF5

NODAL

OTX2

PAX3

PAX4

PAX6

PAX7

PDX1

PROX1

REX1

TAT

ZIC3

HEK293T

E. coli

HEK293

CHO

Sf9

N-His

His-tag

Tag Free

N-GST and C-His

His-Cam-tag

N-GST and C-HIS

N-T7

Druggable Genome

Secreted Protein

Transmembrane

Transcription Factors

ES Cell Differentiation/IPS

Stem cell - Pluripotency

Protease

Protein Kinase

Phosphatase

Adult stem cells

Embryonic stem cells

Induced pluripotent stem cells

Cancer stem cells

Ion Channels: Other

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - JAK/STAT signaling pathway

Nuclear Hormone Receptor

Stem cell relevant signaling - Wnt Signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

GPCR

Suitable for use in ELISA and Western blots.

Ion Channels: Potassium

P450

Ion Channels: Cys-loop Receptors

Suitable for use in ELISA and Western blot.

Ion Channels: Sodium

Ion Channels: Transient receptor potential

Suitable for use in ELISA

ELISA.
Western Blot.

000.

ELISA.
Lateral Flow.

ELISA.
Western Blot.

Colloidal Gold and Latex Beads.

ELISA.

ELISA.
Western blot.
Lateral flow.

Suitable in ELISA

Suitable in Western Blot with a suggested dilution of 1:1

Western blot

Western blot and Immunochromatographic assays.

Western blot and immunochromatographic assays.

0.01 units Xanthine oxidase.
2. Equilibrate to 25°C and monitor at A550nm until the value is constant using a spectrophotometer.
3. Add 20 µl of recombinant SOD2 protein to 50 µg/ml in assay buffer. 4. Mix by inversion and record the increase at A550nm for 5 minutes.

0.033% (w/v) bovine serum albumin.
2. Equilibrate to 37°C and monitor at A340nm until the value is constant using a spectrophotometer
3. Add 50ul of recombinant LDHA protein in various concentrations (0.1ug

0.1 mg/ml Ovalbumin

0.1% (w/v) insulin

0.12 mM beta-nicotinamide adenine dinucleotide

0.5 mM EDTA

0.5ug) in assay buffer.
4. Mix by inversion and record the increase at A340nm for 5 minutes.

0.9 mM Xanthine

0.9ng) in assay buffer and equilibrate to 37C for 10 minutes. (Assay buffer: 50mM Tris-HCl

1 mM DTT

10 mM Tris-HCl

10mM pNPP.
2. Pre-warm reaction mix in heat block at 37°C.
3. Add recombinant PPP1CA solution with various concentrations (2ug

1mM DTT

1mM MnCl2

2 mM dithiothreitol

2.3 mM Pyruvate

2mM DTT

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

3ug) in 200ul reaction buffer.
4. Mix by inversion and incubate at 37°C for 10 minutes.
5. Stop the reaction by adding the 800ul of 0.1N NaOH.
6. Record the absorbance at 405nm.

5.5 mM Ethylendiaminetetraacetic acid

50mM NaCl

63mM sodium phosphate

655077)
- Fluorescent plate reader (PerkinElmer

66 mM Cytochrom-C

ELISA (0.5 µg/ml).
Western blot.

ELISA.
Western Blot.

ELISA.
Western Blot.
Lateral Flow.

ELISA.
Western blot: 1/1000.
Colloidal Gold and Latex Beads.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Western Blot Control.
ATPase activity.
This protein has ATPase activity at the time of manufacture of 2.3 µM Phosphate liberated/hr/µg protein in a 200 µl reaction at 37°C (pH 8) in the presence of 20 µl of 1mM ATP using Malachite Green Assay.
Binding Assay.
ELISA reference standard.

Antigen in ELISA and Western blots.

BML-SE211)
- 96 Well Polystyrene Microplate

Cat.No

Citric acid 0.055 g

Colloidal Gold and Western Blot.
Not recommended for Latex Beads.

D - glucose 2.05g in DW100 ml)
2. Centrifuge at 15000 rpm for 10 minutes and wash four times with PBS.
3. Dilute packed cells in a 0.5 mg/ml trypsin - EDTA solution to give 4% red cell suspension.
4. Incubate for 1h at 37°C and wash four times with PBS.
5 . Dilute packed cells in PBS to give 4% red cell suspension.
6 . Load 50ul of 0.5% BSA - in - 0.15M - NaCl solution and 25ul of 4% Red Cell - in - PBS in u shaped wells.
7 . Add 25ul of serial diluted galectin protein in PBS to each well plate . (Round bottom 96 well plate)
8 . Incubate for 30min at room temperature to observe visible agglutination.

ELISA and Western blot Immunoassay

ELISA and Western blot.

ELISA.
Western blot.

FACS

Ion Channels: ATP Receptors

Protocol: 1. Prepare a 100ul of recombinant PGP9.5 protein with various concentrations (0.48ng

Protocol: Activity Assay
1. Prepare a 180 µl assay buffer into a suitable container and pre-chill on ice before use: The concentrations are 54 mM Potassium Phosphate

Protocol: Activity Assay
1. Prepare a 1.5 ml reaction mix into a suitable container

Protocol: Activity Assay
1. Prepare a 200 ul reaction mix into a suitable container : The final concentrations are 10mM Tris (pH 7.5)

Protocol: Activity Assay
1. Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use:
The final concentrations are 200 mM Tris-HCl

Protocol: Biological Assay :
1. Mix equal volumes of human blood and Alsever’s solution (pH 7.0). (Alsever’s solution: NaCl 0.42 g

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Sodium citric aci d 0.8g

Suitable for ELISA

Suitable for ELISA.

Suitable for use in ELISA and Western blot

Suitable for use in ELISA and Western blot immunoassay.

Suitable in ELISA.

VICTOR X3)

Western Blot and ELISA.

adjust to pH 7.5 at 37°C and pre-chill on ice before use: The final concentrations are 100 mM Sodium phosphate

and 20nM chymotrypsin.
2. Add 10 ul of recombinant Cyclophilin A protein with 1 ug in assay buffer.
3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
4. Add 20 ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
5. Record the increase in A405 nm for 30 minutes at 25°C.

black (greiner bio-one

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbecco's PBS).

pH 7.4).
For immunization purposes

pH 8.0

pH 8.0.)

2. Add 50ul of 1uM Ubiquitin-AMC.

3. Read at excitation wavelengths 355nm and emission 460nm for 5 minutes.
- Ubiquitin-AMC (Enzo

reduced form

the solution can be further dialyzed against PBS (phosphate buffered saline

Pathways in cancer

MAPK signaling pathway

TGF-beta signaling pathway

Regulation of actin cytoskeleton

Melanoma

Hedgehog signaling pathway

Cytokine-cytokine receptor interaction

Endocytosis

Basal cell carcinoma

Hematopoietic cell lineage

Acute myeloid leukemia

Pancreatic cancer

Bladder cancer

Endometrial cancer

ErbB signaling pathway

Huntington's disease

Melanogenesis

Neurotrophin signaling pathway

Cell cycle

Colorectal cancer

Focal adhesion

Gap junction

Glioma

Non-small cell lung cancer

Prostate cancer

Wnt signaling pathway

Chronic myeloid leukemia

Adherens junction

Cell adhesion molecules (CAMs)

Jak-STAT signaling pathway

Maturity onset diabetes of the young

Small cell lung cancer

Thyroid cancer

Pathogenic Escherichia coli infection

Toll-like receptor signaling pathway

Cysteine and methionine metabolism

Metabolic pathways

Phenylalanine

Phenylalanine metabolism

Type II diabetes mellitus

Tyrosine metabolism

Ubiquinone and other terpenoid-quinone biosynthesis

tyrosine and tryptophan biosynthesis

2

1

Yes

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