Recombinant Proteins

Proteins

Recombinant Proteins

Native Proteins

Mutant Proteins

Human

Mouse

Escherichia coli

SARS-CoV-2

Rat

Cynomolgus

Rhesus

Bovine

Staphylococcus aureus

Rabbit

Blomia tropicalis

Chicken

viral

2019-nCoV

Dermatophagoides pteronyssinus

Orf Virus

Saccharomyces cerevisiae

Streptococcus Pyogenes

Protozoan

Schistosoma

Streptococcus

Hepatitis B Virus

Mycobacteria

A. victoria

C. elegans

Cavia porcellus

Dermatophagoides farinae

Drosophila

HIV

High Gc Gram+

Human, Rat

Monkey

Photinus Pyr.

Porcine

Streptococcus sp.

Yeast

Adeno-Associated Virus 6

Adenovirus 6

Alternaria alternata

Apicomplexan

B.subtilis

Bacterial

Betula pendula

Borrelia burgdorferi

Campylobacter jejuni

Canine

Carassiusauratus

Chlorocebus aethiops

D. aromatica

Drosophila melanogaster

E.coli

Enterobacteria phage T4

Escherichia phage T7

Feline

Hamster

Hirudo medicinalis

Human herpesvirus

Human papillomavirus

Humon

Humulus japonicus

Klebsiella pneumoniae

Lachnospiraceae bacterium ND2006

Lepidoglyphus destructor

Leptotrichia wadei

Macaca fascicularis

Marmoset

Mucor hiemalis

Mus musculus

Pig

Proteobacteria

S. hygroscopicus

SIV

Serratia marcescens

Sheep

Spirochetes

Staphylococcus aureus subsp.

Streptomyces avidinii

Streptomyces plicatus

T. fusca

TEV

Tobacco etch virus

Vaccinia virus

Vacciniavirus

Vibrio cholerae

VEGFA

PD-L1

MESOTHELIN

PD1

EPCAM

ALPHA 1 ANTITRYPSIN

NCALD

IL6

FGF1

TACD2

HER2

IL2

SNCA

IRF5

NDRG2

S PROTEIN

FGF2

CD22

FGFR1

IGF1

IL4

ISOCITRATE DEHYDROGENASE

PQBP1

UBE2D3

ALKALINE PHOSPHATASE

ALPHA SYNUCLEIN

CPNE1

CSF2

HMGA1

MAX

RIBONUCLEASE INHIBITOR

TNF ALPHA

TPM1

CD19

CD7

CTLA4

EGFR

FSTL1

ICAM2

IL22

IL33

M-CSF

MIF

MORTALITY FACTOR 4 LIKE 2

BCMA

CD38

MTH1

PRKAR1B

UBE2I

AURORA A

CD30

CD36

CD3E

CD47

EGF

FGL1

IL12B

MYOSIN

S100A8

ADIPOQ

CD137

CD70

CNTF

FHL1

GALE

IL8

LIF

PCBP2

PCSK9

PGF

RNA5-8SN2

S100 CALCIUM BINDING PROTEIN A13

SOD2

SULT1A1

TNF

ALDOLASE

ARSA

B3GALT5

BETA 2 MICROGLOBULIN

CATHEPSIN B

CD83

CUTA

DYNLL1

FHL2

FKBP2

GLUTAMINE SYNTHETASE

GM CSF

HMG1

IL1B

MBNL1

MICA

MYELIN BASIC PROTEIN

NDUF3

TROPOMYOSIN 3

14-3-3 ZETA

ACRV1

AKT1

ASPARTATE BETA HYDROXYLASE

BDNF

CD16

HEK293T

E. coli

HEK293

CHO

Sf9

Insect

Pichia pastoris

Baculovirus

Pichia

Hi-5 insect

S. cerevisiae

Mammalian

Mouse

Other

Sf9 insect

human serum

A549

H. vulgare

HeLa-229

Human

Yeast

C-Myc/DDK

C-MYC/DDK

His-tag

C-His

N-His

Tag Free

C-Fc

N-GST and C-His

C-6His

C-Human Fc

C-DDK

C-6×His

N-GST and C-HIS

N-GST

C-DDK/His

C-His-Avi

Myc-DDK

C-Fc&His

C-FLAG

N-6His

C-Mouse Fc

N-His-ABP (Albumin-Binding Protein)

N-Human Fc

C-mFc

C-HIS

N-His-SUMO

C-Fc-Avi

His-Tag

N-His&C-His

C-6His-Avi

C-8His

C-Mouse Fc and 6×His

T7-tag

C-hFc

N-T7&C-His

N-Trx&His

C-Avi-6His

N-His-Trx

C-Human Fc and 6×His

C-10His

N-Fc

N-GST, C-His

hIgG-His-Tag

N-HIS

N-His&SUMO

hIgG-His-tag

C- 6×His

N-6×His

N-GST&His

Tag-free

C-6xHis

C-Avi&6His

hIgG-Tag

C-AVI&His

C-Fc&AVI

N-8His

N-Avi

N-His PDCD1(Pro21-Val170)

N-His/DDK

N-Mouse Fc and C-6×His

C-6×His tag and C-Human Fc

C-TEV-8His

C-mouse Fc

His-Cam-tag

His-MBP-tag

N-6His-Avi

N-His&C-Fc

N-His&T7

N-His-GST

N-MARI

N-Trx

C-Fc/His

C-Flag

C-hFc&His

His-tagged, myc-tagged

N-DDK

N-His&Flag

N-mFc

C-3×Flag Tag

C-6xHis/AVI

C-7×His

C-Avi&His

C-DDK-His

C-FC

C-Fc-Flag&C-Fc-6His

C-HUMAN

CaM-tag

Calmodulin-tag

Fc chimera

Myc-His-tag

N-6His-Flag

N-6xHis/AVI

N-8His&N-FLAG

N-FC

N-FLAG

N-Fc-FLAG

N-His-PDCD1(Pro21-Val170)

N-Mouse Fc

N-TrxA

N-terminal His

N-terminal His-PDCD1(Pro21-Val170) tag

TrxA

C-AVI

C-AVI-PLUS

C-Avi

C-Avi-10His

C-Avi-His

C-DDK/10xHis

C-DDK/6xHis

C-FC-6HIS&C-FC-Flag

C-FC/6xHis

C-FLAG-6His

C-FLAG-Fc

C-Fc Human

C-Fc Mouse

C-Flag,C-6His

C-GST

C-HIS/C

C-HIS/DDK

C-His&AVI

C-His&Flag

C-His&HA&AVI

C-His/AVIplus

C-His/DDK

C-Human Fc, Avi

C-Human Fc-Avi

C-MIgG2a

C-hIgG1 Fc

C-hIgG2 Fc

C-hIgG4 Fc

C-hIgG4P Fc

C-mFC

C-mFc&His

C-mIgG2AFc

C-mIgG2a Fc

C-mIgG2aFc

C-terminal DDK

C-terminal His

DDK-tag

His tag

N-10*His

N-10His

N-10His-Flag

N-6His&Avi

N-6His, C-6His

N-6His-FLAG

N-6xHis

N-8His-Flag

N-AVI

N-AVI&His

N-Avi&6His

N-BBP&His

N-DDK/His

N-FUSION

N-Flag&C-His

N-Flag&His

N-GFP

N-GST(CKM) and N-His(CKB)

N-HIS and C-AVIPLUS

N-His&MBP

N-PROTEIN

N-T7

N-TRX

N-TRXA

N-Trx-His

N-hFc

N-terminal His-tag

N-terminal murine Fc Tag

No

Tag free

APC

Biotin

PE

FITC

Sepharose

AP

HRP

Agarose

Yes

MVPro

SDS PAGE

Druggable Genome

Transmembrane

Secreted Protein

Transcription Factors

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

Phosphatase

Adult stem cells

Ion Channels: Other

Embryonic stem cells

Cancer stem cells

Nuclear Hormone Receptor

Induced pluripotent stem cells

P450

GPCR

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - Wnt Signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Potassium

ELISA.

Ion Channels: Cys-loop Receptors

Ion Channels: Glutamate Receptors

Ion Channels: ATP Receptors

Ion Channels: Transient receptor potential

Ion Channels: Sodium

Suitable for use in ELISA and Western blots.

Suitable for use in ELISA and Western blot.

ELISA.
Western Blot.

Suitable for ELISA and Western blot.

ELISA.
Western Blot.

Suitable for use in ELISA

Functional Assays.

ELISA

FACS

Ion Channels: Cyclic nucleotide gated

Suitable for use in ELISA.

000.

ELISA.
Lateral Flow.

ELISA.

ELISA.
Western Blot.

Colloidal Gold and Latex Beads.

ELISA and Western blot.

ELISA.
Functional Assays.

ELISA.
Western blot.
Lateral flow.

ELISA: 0.5-1.0 µg/ml coating concentration.
After thawing

ELISA: 1.25µg/ml.

Suitable in ELISA

Suitable in Western Blot with a suggested dilution of 1:1

Western blot

Western blot and Immunochromatographic assays.

Western blot and immunochromatographic assays.

mix thoroughly by vortexing before use.

0.01 units Xanthine oxidase.
2. Equilibrate to 25°C and monitor at A550nm until the value is constant using a spectrophotometer.
3. Add 20 µl of recombinant SOD2 protein to 50 µg/ml in assay buffer. 4. Mix by inversion and record the increase at A550nm for 5 minutes.

0.033% (w/v) bovine serum albumin.
2. Equilibrate to 37°C and monitor at A340nm until the value is constant using a spectrophotometer
3. Add 50ul of recombinant LDHA protein in various concentrations (0.1ug

0.1 mg/ml Ovalbumin

0.1% (w/v) insulin

0.12 mM beta-nicotinamide adenine dinucleotide

0.5 mM EDTA

0.5ug) in assay buffer.
4. Mix by inversion and record the increase at A340nm for 5 minutes.

0.9 mM Xanthine

0.9ng) in assay buffer and equilibrate to 37C for 10 minutes. (Assay buffer: 50mM Tris-HCl

000 and 30

000 and 50

000 daltons and a doublet band at approximately 28

000 daltons that are not glycosylated.

1 mM DTT

10 mM Tris-HCl

10mM pNPP.
2. Pre-warm reaction mix in heat block at 37°C.
3. Add recombinant PPP1CA solution with various concentrations (2ug

1mM DTT

1mM MnCl2

2 mM dithiothreitol

2.3 mM Pyruvate

2mM DTT

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

3ug) in 200ul reaction buffer.
4. Mix by inversion and incubate at 37°C for 10 minutes.
5. Stop the reaction by adding the 800ul of 0.1N NaOH.
6. Record the absorbance at 405nm.

5.5 mM Ethylendiaminetetraacetic acid

50mM NaCl

63mM sodium phosphate

655077)
- Fluorescent plate reader (PerkinElmer

66 mM Cytochrom-C

ELISA (0.5 µg/ml).
Western blot.

ELISA and Western blot.
Using an antigen Capture ELISA

ELISA.
Western blot.

ELISA.
Western Blot.
Lateral Flow.

ELISA.
Western blot: 1/1000.
Colloidal Gold and Latex Beads.

ELISA: 0.6-1.25 µg/ml.
Gel Super Shift Assays.

ELISA: SA1000 May be used as a standard with SM2216P or SM2228B.
Functional Assays.

ELISA: When coating use 10 ng-1 µg/well.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Western Blot Control.
ATPase activity.
This protein has ATPase activity at the time of manufacture of 2.3 µM Phosphate liberated/hr/µg protein in a 200 µl reaction at 37°C (pH 8) in the presence of 20 µl of 1mM ATP using Malachite Green Assay.
Binding Assay.
ELISA reference standard.

Antigen in ELISA and Western blots.

BML-SE211)
- 96 Well Polystyrene Microplate

Cat.No

Citric acid 0.055 g

Colloidal Gold and Western Blot.
Not recommended for Latex Beads.

D - glucose 2.05g in DW100 ml)
2. Centrifuge at 15000 rpm for 10 minutes and wash four times with PBS.
3. Dilute packed cells in a 0.5 mg/ml trypsin - EDTA solution to give 4% red cell suspension.
4. Incubate for 1h at 37°C and wash four times with PBS.
5 . Dilute packed cells in PBS to give 4% red cell suspension.
6 . Load 50ul of 0.5% BSA - in - 0.15M - NaCl solution and 25ul of 4% Red Cell - in - PBS in u shaped wells.
7 . Add 25ul of serial diluted galectin protein in PBS to each well plate . (Round bottom 96 well plate)
8 . Incubate for 30min at room temperature to observe visible agglutination.

ELISA and Western blot Immunoassay

ELISA and Western blot.
Glycosylation is heterogeneous and results in several immunoreactive products on Western blots that range from approximately 40

ELISA.
Western Blotting.

ELISA.
Western blot.

Flow Cytometry.

Flow Cytometry: Use 10 µl of Neat antibody to label 10e6 cells in 100 µl.
RA021APC is designed as a secondary reagent for use in Flow Cytometry in conjunction with biotinylated primary antibodies.
RA021APC conjugates show negligible non-specific binding to non-biotinylated macromolecules

Flow Cytometry: Use 10µl of the suggested working dilution to label 10e6 cells or 100µl of whole blood.

Functional Assays.

Functional Assays.
In vitro Assay.

Functional assays.

Immunoassay.

In vitro Assay.
Functional Assays.

Protocol: 1. Prepare a 100ul of recombinant PGP9.5 protein with various concentrations (0.48ng

Protocol: Activity Assay
1. Prepare a 180 µl assay buffer into a suitable container and pre-chill on ice before use: The concentrations are 54 mM Potassium Phosphate

Protocol: Activity Assay
1. Prepare a 1.5 ml reaction mix into a suitable container

Protocol: Activity Assay
1. Prepare a 200 ul reaction mix into a suitable container : The final concentrations are 10mM Tris (pH 7.5)

Protocol: Activity Assay
1. Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use:
The final concentrations are 200 mM Tris-HCl

Protocol: Biological Assay :
1. Mix equal volumes of human blood and Alsever’s solution (pH 7.0). (Alsever’s solution: NaCl 0.42 g

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Recommended for use in standard solid phase immunoassays.

Sodium citric aci d 0.8g

Suitable for ELISA

Suitable for ELISA.

Suitable for use in ELISA and Western blot

Suitable for use in ELISA and Western blot immunoassay.

Suitable for use in Western blot.

Suitable in ELISA.

Tested as solid-phase coating antigen in ELISA with anti-HBsAg antibodies.

Tested in ELISA with anti-HBsAg antibodies.

The DYKDDDDK peptide (Flag) is used as a fusion tag for immunoaffinity chromatography.

Use in standard solid phase immunoassays.

VICTOR X3)

Western Blot and ELISA.

Western Blot.
ELISA.

Western Blot
ELISA

adjust to pH 7.5 at 37°C and pre-chill on ice before use: The final concentrations are 100 mM Sodium phosphate

and 20nM chymotrypsin.
2. Add 10 ul of recombinant Cyclophilin A protein with 1 ug in assay buffer.
3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
4. Add 20 ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
5. Record the increase in A405 nm for 30 minutes at 25°C.

and therefore gives very low backgrounds.

black (greiner bio-one

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbecco's PBS).

each lot is tested against a reference material for both IGM and IgG activity.

pH 7.4).
For immunization purposes

pH 8.0

pH 8.0.)

2. Add 50ul of 1uM Ubiquitin-AMC.

3. Read at excitation wavelengths 355nm and emission 460nm for 5 minutes.
- Ubiquitin-AMC (Enzo

reduced form

the solution can be further dialyzed against PBS (phosphate buffered saline

Metabolic pathways

Cytokine-cytokine receptor interaction

Pathways in cancer

MAPK signaling pathway

Focal adhesion

Regulation of actin cytoskeleton

Chemokine signaling pathway

Jak-STAT signaling pathway

Endocytosis

Natural killer cell mediated cytotoxicity

Neurotrophin signaling pathway

T cell receptor signaling pathway

Hematopoietic cell lineage

Purine metabolism

Toll-like receptor signaling pathway

Alzheimer's disease

Ubiquitin mediated proteolysis

Cell adhesion molecules (CAMs)

Insulin signaling pathway

Axon guidance

Huntington's disease

Cell cycle

Wnt signaling pathway

Leukocyte transendothelial migration

ErbB signaling pathway

Prostate cancer

Apoptosis

Pancreatic cancer

Melanoma

Pyrimidine metabolism

Tight junction

Progesterone-mediated oocyte maturation

Fc epsilon RI signaling pathway

Oocyte meiosis

Spliceosome

Renal cell carcinoma

Adherens junction

Colorectal cancer

GnRH signaling pathway

TGF-beta signaling pathway

NOD-like receptor signaling pathway

Lysosome

Antigen processing and presentation

Parkinson's disease

Calcium signaling pathway

Fc gamma R-mediated phagocytosis

RIG-I-like receptor signaling pathway

Hypertrophic cardiomyopathy (HCM)

B cell receptor signaling pathway

Chronic myeloid leukemia

Glioma

VEGF signaling pathway

Glycolysis / Gluconeogenesis

Dilated cardiomyopathy

Epithelial cell signaling in Helicobacter pylori infection

Systemic lupus erythematosus

p53 signaling pathway

Adipocytokine signaling pathway

Small cell lung cancer

Bladder cancer

PPAR signaling pathway

Gap junction

Complement and coagulation cascades

Cytosolic DNA-sensing pathway

Melanogenesis

Acute myeloid leukemia

Vascular smooth muscle contraction

Arginine and proline metabolism

Type I diabetes mellitus

Endometrial cancer

Long-term potentiation

Non-small cell lung cancer

Oxidative phosphorylation

Amyotrophic lateral sclerosis (ALS)

Pathogenic Escherichia coli infection

Autoimmune thyroid disease

mTOR signaling pathway

Viral myocarditis

Glutathione metabolism

Long-term depression

Metabolism of xenobiotics by cytochrome P450

Phosphatidylinositol signaling system

Amino sugar and nucleotide sugar metabolism

Drug metabolism - cytochrome P450

Allograft rejection

Graft-versus-host disease

ECM-receptor interaction

Fructose and mannose metabolism

Cardiac muscle contraction

Type II diabetes mellitus

Tyrosine metabolism

Ribosome

Valine

Proteasome

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Inositol phosphate metabolism

Prion diseases

Neuroactive ligand-receptor interaction

Drug metabolism - other enzymes

leucine and isoleucine degradation

Thyroid cancer

Glycerophospholipid metabolism

RNA degradation

Cysteine and methionine metabolism

Pyruvate metabolism

Tryptophan metabolism

Alanine

Hedgehog signaling pathway

Regulation of autophagy

aspartate and glutamate metabolism

Retinol metabolism

Fatty acid metabolism

Starch and sucrose metabolism

Vibrio cholerae infection

Pentose phosphate pathway

Base excision repair

Primary immunodeficiency

Arachidonic acid metabolism

Nitrogen metabolism

Asthma

Porphyrin and chlorophyll metabolism

Androgen and estrogen metabolism

Glycerolipid metabolism

Nucleotide excision repair

SNARE interactions in vesicular transport

Galactose metabolism

Butanoate metabolism

Citrate cycle (TCA cycle)

Propanoate metabolism

Basal cell carcinoma

DNA replication

Glycine

serine and threonine metabolism

Aminoacyl-tRNA biosynthesis

Lysine degradation

Histidine metabolism

beta-Alanine metabolism

Nicotinate and nicotinamide metabolism

Sphingolipid metabolism

Phenylalanine metabolism

Olfactory transduction

Notch signaling pathway

RNA polymerase

Dorso-ventral axis formation

Selenoamino acid metabolism

Ether lipid metabolism

Maturity onset diabetes of the young

Sulfur metabolism

Glyoxylate and dicarboxylate metabolism

Homologous recombination

Linoleic acid metabolism

Mismatch repair

Riboflavin metabolism

Pentose and glucuronate interconversions

Terpenoid backbone biosynthesis

Pantothenate and CoA biosynthesis

Basal transcription factors

Other glycan degradation

Renin-angiotensin system

Glycosaminoglycan degradation

N-Glycan biosynthesis

One carbon pool by folate

Ascorbate and aldarate metabolism

Glycosphingolipid biosynthesis - lacto and neolacto series

Folate biosynthesis

Primary bile acid biosynthesis

Taste transduction

C21-Steroid hormone metabolism

Limonene and pinene degradation

alpha-Linolenic acid metabolism

Glycosphingolipid biosynthesis - globo series

Synthesis and degradation of ketone bodies

O-Glycan biosynthesis

Thiamine metabolism

Biosynthesis of unsaturated fatty acids

Methane metabolism

Non-homologous end-joining

Heparan sulfate biosynthesis

Keratan sulfate biosynthesis

Circadian rhythm - mammal

Steroid biosynthesis

ABC transporters

Fatty acid elongation in mitochondria

Taurine and hypotaurine metabolism

Glycosphingolipid biosynthesis - ganglio series

Ubiquinone and other terpenoid-quinone biosynthesis

Chondroitin sulfate biosynthesis

Phenylalanine

leucine and isoleucine biosynthesis

tyrosine and tryptophan biosynthesis

Glycosylphosphatidylinositol(GPI)-anchor biosynthesis

Protein export

Caffeine metabolism

Vitamin B6 metabolism

Fatty acid biosynthesis

Cyanoamino acid metabolism

Lysine biosynthesis

D-Glutamine and D-glutamate metabolism

D-Arginine and D-ornithine metabolism

Biotin metabolism

O-Mannosyl glycan biosynthesis

Lipoic acid metabolism

E. coli

Human

Human 293

P.Pichia

Insect

Baculovirus

HEK293

Pichia pastoris

293F

CHO Stable

Expi293

Mammalian

1

2

3

Yes

In Stock