Recombinant Proteins

Proteins

Recombinant Proteins

E. coli

Druggable Genome

Transmembrane

Secreted Protein

Transcription Factors

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

Phosphatase

Adult stem cells

Ion Channels: Other

Embryonic stem cells

Cancer stem cells

Nuclear Hormone Receptor

Induced pluripotent stem cells

P450

GPCR

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - Wnt Signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Potassium

ELISA.

Ion Channels: Cys-loop Receptors

Ion Channels: Glutamate Receptors

Ion Channels: ATP Receptors

Ion Channels: Transient receptor potential

Ion Channels: Sodium

Suitable for use in ELISA and Western blots.

Suitable for use in ELISA and Western blot.

ELISA.
Western Blot.

Suitable for ELISA and Western blot.

ELISA.
Western Blot.

Suitable for use in ELISA

Functional Assays.

ELISA

FACS

Ion Channels: Cyclic nucleotide gated

Suitable for use in ELISA.

000.

ELISA.
Lateral Flow.

ELISA.

ELISA.
Western Blot.

Colloidal Gold and Latex Beads.

ELISA and Western blot.

ELISA.
Functional Assays.

ELISA.
Western blot.
Lateral flow.

ELISA: 0.5-1.0 µg/ml coating concentration.
After thawing

ELISA: 1.25µg/ml.

Suitable in ELISA

Suitable in Western Blot with a suggested dilution of 1:1

Western blot

Western blot and Immunochromatographic assays.

Western blot and immunochromatographic assays.

mix thoroughly by vortexing before use.

0.01 units Xanthine oxidase.
2. Equilibrate to 25°C and monitor at A550nm until the value is constant using a spectrophotometer.
3. Add 20 µl of recombinant SOD2 protein to 50 µg/ml in assay buffer. 4. Mix by inversion and record the increase at A550nm for 5 minutes.

0.033% (w/v) bovine serum albumin.
2. Equilibrate to 37°C and monitor at A340nm until the value is constant using a spectrophotometer
3. Add 50ul of recombinant LDHA protein in various concentrations (0.1ug

0.1 mg/ml Ovalbumin

0.1% (w/v) insulin

0.12 mM beta-nicotinamide adenine dinucleotide

0.5 mM EDTA

0.5ug) in assay buffer.
4. Mix by inversion and record the increase at A340nm for 5 minutes.

0.9 mM Xanthine

0.9ng) in assay buffer and equilibrate to 37C for 10 minutes. (Assay buffer: 50mM Tris-HCl

000 and 30

000 and 50

000 daltons and a doublet band at approximately 28

000 daltons that are not glycosylated.

000 or dot blot)

000)

000) and Immunohistochemistry (1/200-1/1

000) as well as other Glucose Oxidase-biotin-avidin based enzymatic assays.

000) as well as other phosphatase-Biotin/biotin based enzymatic assays.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Biotin.

000-1/200

000-1/32

000-1/40

000-1/5

1 mM DTT

1/500-1/2

10 mM Tris-HCl

10mM pNPP.
2. Pre-warm reaction mix in heat block at 37°C.
3. Add recombinant PPP1CA solution with various concentrations (2ug

1mM DTT

1mM MnCl2

2 mM dithiothreitol

2.3 mM Pyruvate

2mM DTT

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

3ug) in 200ul reaction buffer.
4. Mix by inversion and incubate at 37°C for 10 minutes.
5. Stop the reaction by adding the 800ul of 0.1N NaOH.
6. Record the absorbance at 405nm.

5.5 mM Ethylendiaminetetraacetic acid

500)

50mM NaCl

63mM sodium phosphate

655077)
- Fluorescent plate reader (PerkinElmer

66 mM Cytochrom-C

ELISA (0.5 µg/ml).
Western blot.

ELISA and Western blot.
Using an antigen Capture ELISA

ELISA.
Western blot.

ELISA.
Western Blot.
Lateral Flow.

ELISA.
Western blot: 1/1000.
Colloidal Gold and Latex Beads.

ELISA: 0.6-1.25 µg/ml.
Gel Super Shift Assays.

ELISA: SA1000 May be used as a standard with SM2216P or SM2228B.
Functional Assays.

ELISA: When coating use 10 ng-1 µg/well.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Western Blot Control.
ATPase activity.
This protein has ATPase activity at the time of manufacture of 2.3 µM Phosphate liberated/hr/µg protein in a 200 µl reaction at 37°C (pH 8) in the presence of 20 µl of 1mM ATP using Malachite Green Assay.
Binding Assay.
ELISA reference standard.

Antigen in ELISA and Western blots.

BML-SE211)
- 96 Well Polystyrene Microplate

Cat.No

Citric acid 0.055 g

Colloidal Gold and Western Blot.
Not recommended for Latex Beads.

D - glucose 2.05g in DW100 ml)
2. Centrifuge at 15000 rpm for 10 minutes and wash four times with PBS.
3. Dilute packed cells in a 0.5 mg/ml trypsin - EDTA solution to give 4% red cell suspension.
4. Incubate for 1h at 37°C and wash four times with PBS.
5 . Dilute packed cells in PBS to give 4% red cell suspension.
6 . Load 50ul of 0.5% BSA - in - 0.15M - NaCl solution and 25ul of 4% Red Cell - in - PBS in u shaped wells.
7 . Add 25ul of serial diluted galectin protein in PBS to each well plate . (Round bottom 96 well plate)
8 . Incubate for 30min at room temperature to observe visible agglutination.

ELISA (1/20

ELISA (1/8

ELISA and Western blot Immunoassay

ELISA and Western blot.
Glycosylation is heterogeneous and results in several immunoreactive products on Western blots that range from approximately 40

ELISA.
Western Blotting.

ELISA.
Western blot.

Flow Cytometry.

Flow Cytometry: Use 10 µl of Neat antibody to label 10e6 cells in 100 µl.
RA021APC is designed as a secondary reagent for use in Flow Cytometry in conjunction with biotinylated primary antibodies.
RA021APC conjugates show negligible non-specific binding to non-biotinylated macromolecules

Flow Cytometry: Use 10µl of the suggested working dilution to label 10e6 cells or 100µl of whole blood.

Functional Assays.

Functional Assays.
In vitro Assay.

Functional assays.

Immunoassay.

In vitro Assay.
Functional Assays.

Protocol: 1. Prepare a 100ul of recombinant PGP9.5 protein with various concentrations (0.48ng

Protocol: Activity Assay
1. Prepare a 180 µl assay buffer into a suitable container and pre-chill on ice before use: The concentrations are 54 mM Potassium Phosphate

Protocol: Activity Assay
1. Prepare a 1.5 ml reaction mix into a suitable container

Protocol: Activity Assay
1. Prepare a 200 ul reaction mix into a suitable container : The final concentrations are 10mM Tris (pH 7.5)

Protocol: Activity Assay
1. Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use:
The final concentrations are 200 mM Tris-HCl

Protocol: Biological Assay :
1. Mix equal volumes of human blood and Alsever’s solution (pH 7.0). (Alsever’s solution: NaCl 0.42 g

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Recommended for use in standard solid phase immunoassays.

Sodium citric aci d 0.8g

Suitable for ELISA

Suitable for ELISA.

Suitable for Immunoblotting (Western or Dot blot

Suitable for immunoblotting (Western: 1/10

Suitable for use in ELISA and Western blot

Suitable for use in ELISA and Western blot immunoassay.

Suitable for use in Western blot.

Suitable in ELISA.

Tested as solid-phase coating antigen in ELISA with anti-HBsAg antibodies.

Tested in ELISA with anti-HBsAg antibodies.

The DYKDDDDK peptide (Flag) is used as a fusion tag for immunoaffinity chromatography.

Use in standard solid phase immunoassays.

VICTOR X3)

Western Blot and ELISA.

Western Blot.
ELISA.

Western Blot
ELISA

adjust to pH 7.5 at 37°C and pre-chill on ice before use: The final concentrations are 100 mM Sodium phosphate

and 20nM chymotrypsin.
2. Add 10 ul of recombinant Cyclophilin A protein with 1 ug in assay buffer.
3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
4. Add 20 ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
5. Record the increase in A405 nm for 30 minutes at 25°C.

and therefore gives very low backgrounds.

black (greiner bio-one

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbecco's PBS).

each lot is tested against a reference material for both IGM and IgG activity.

immunoperoxidase electron microscopy and immunochemistry (1/1

pH 7.4).
For immunization purposes

pH 8.0

pH 8.0.)

2. Add 50ul of 1uM Ubiquitin-AMC.

3. Read at excitation wavelengths 355nm and emission 460nm for 5 minutes.
- Ubiquitin-AMC (Enzo

reduced form

the solution can be further dialyzed against PBS (phosphate buffered saline

2