Recombinant Proteins

Proteins

Recombinant Proteins

E. coli

Druggable Genome

Transmembrane

Secreted Protein

Transcription Factors

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

Phosphatase

Adult stem cells

Ion Channels: Other

Embryonic stem cells

Cancer stem cells

Nuclear Hormone Receptor

Induced pluripotent stem cells

P450

GPCR

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - Wnt Signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Potassium

Ion Channels: Cys-loop Receptors

Ion Channels: Glutamate Receptors

Ion Channels: ATP Receptors

Ion Channels: Transient receptor potential

Ion Channels: Sodium

Suitable for use in ELISA and Western blots.

Suitable for use in ELISA and Western blot.

Suitable for ELISA and Western blot.

ELISA.
Western Blot.

Suitable for use in ELISA

ELISA

ELISA.
Western Blot.

Ion Channels: Cyclic nucleotide gated

Suitable for use in ELISA.

000.

ELISA.
Lateral Flow.

ELISA.
Western Blot.

Colloidal Gold and Latex Beads.

ELISA and Western blot.

ELISA.

ELISA.
Western blot.
Lateral flow.

ELISA: 0.5-1.0 µg/ml coating concentration.
After thawing

Suitable in ELISA

Suitable in Western Blot with a suggested dilution of 1:1

Western blot

Western blot and Immunochromatographic assays.

Western blot and immunochromatographic assays.

mix thoroughly by vortexing before use.

000 and 30

000 and 50

000 daltons and a doublet band at approximately 28

000 daltons that are not glycosylated.

000) and Immunohistochemistry (1/200-1/1

000) as well as other phosphatase-Biotin/biotin based enzymatic assays.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Biotin.

000-1/32

1/500-1/2

10 mM Tris-HCl

2 mM dithiothreitol

500)

ELISA (0.5 µg/ml).
Western blot.

ELISA and Western blot.
Using an antigen Capture ELISA

ELISA.
Western blot.

ELISA.
Western Blot.
Lateral Flow.

ELISA.
Western blot: 1/1000.
Colloidal Gold and Latex Beads.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Antigen in ELISA and Western blots.

Colloidal Gold and Western Blot.
Not recommended for Latex Beads.

ELISA (1/8

ELISA and Western blot Immunoassay

ELISA and Western blot.
Glycosylation is heterogeneous and results in several immunoreactive products on Western blots that range from approximately 40

ELISA.
Western blot.

FACS

Recommended for use in standard solid phase immunoassays.

Suitable for ELISA

Suitable for ELISA.

Suitable for Immunoblotting (Western or Dot blot

Suitable for use in ELISA and Western blot

Suitable for use in ELISA and Western blot immunoassay.

Suitable for use in Western blot.

Suitable in ELISA.

Tested as solid-phase coating antigen in ELISA with anti-HBsAg antibodies.

Tested in ELISA with anti-HBsAg antibodies.

The DYKDDDDK peptide (Flag) is used as a fusion tag for immunoaffinity chromatography.

Use in standard solid phase immunoassays.

Western Blot and ELISA.

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbecco's PBS).

each lot is tested against a reference material for both IGM and IgG activity.

pH 7.4).
For immunization purposes

the solution can be further dialyzed against PBS (phosphate buffered saline

2